Plant promoter from cotton and uses thereof
The present disclosure provides a cotton promoter, designated “p2”, which exhibits promoter activity. Interestingly, the promoter is also influenced by water or salt stress. Deletion analysis reveals upstream elements/motifs in the promoter which influence promoter activity, and sequences that are potentially responsive to salt or water stress.
1. A DNA construct comprising a promoter capable of driving expression of an operably linked heterologous gene of interest, said promoter selected from the group consisting of:
a. a DNA having the sequence as set forth in SEQ ID NO: 1; and
b. a DNA having the DNA sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 9 or SEQ ID NO: 10,
wherein said promoter is operably linked to a heterologous gene fragment.
2. A DNA vector comprising the DNA construct as claimed in claim 1 .
3. A recombinant host cell comprising the DNA construct as claimed in claim 1 , wherein said host cell is of bacterial, fungal, or plant origin.
4. A recombinant host cell comprising the DNA vector as claimed in claim 2 , wherein said host cell is of bacterial or fungal origin.
5. A transgenic plant or part thereof, including seeds, comprising within its genome, a DNA construct comprising a promoter capable of driving expression of an operably linked heterologous gene of interest, said promoter selected from the group consisting of:
a) a DNA having the sequence as set forth in SEQ ID NO: 1; and
b) a DNA having the DNA sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 9 or SEQ ID NO: 10,
wherein said promoter is operably linked to a heterologous gene fragment.
6. The transgenic plant as claimed in claim 5 , wherein said plant is a monocot, or a dicot.
7. A method of producing a transgenic plant, said method comprising:
a) obtaining plant cell;
b) obtaining a DNA construct comprising a promoter operably linked to a heterologous gene of interest, said promoter selected from the group consisting of:
(i) a DNA having the sequence as set forth in SEQ ID NO:1; and
(ii) a DNA having the DNA sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 9 or SEQ ID NO: 10;
(c) transforming said plant cell with said DNA construct of step (b), to obtain a transformed plant cell; and
(d) selecting the transformed plant cell expressing said gene of interest.
8. The method as claimed in claim 7 , wherein said gene of interest expression is constitutive.
9. The method as claimed in claim 7 , wherein said gene of interest expression is regulated in response to salt or water stress.
10. The method as claimed in claim 7 , wherein said gene of interest expression is root specific under conditions of water stress.
11. The method as claimed in claim 7 , wherein said transformation is carried out by a method selected from the group consisting of particle gun bombardment method, microinjection method, and macroinjection method.
12. A method of generating a transgenic plant, said method comprising:
(a) obtaining plant cell;
(b) obtaining a DNA construct comprising a promoter operably linked to a heterologous gene of interest, said promoter selected from the group consisting of:
(i) a DNA having the sequence as set forth in SEQ ID NO: 1; and
(ii) a DNA having the DNA sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 9 or SEQ ID NO: 10,
(c) transforming a host cell with the DNA construct of step (b) to obtain a recombinant host cell;
(d) transforming said plant cell with said recombinant host cell of step (c) to obtain a transformed plant cell; and
(e) selecting the transformed plant cell expressing said gene of interest.
13. The method as claimed in claim 12 , wherein said gene of interest expression is constitutive.
14. The method as claimed in claim 12 , wherein said gene of interest expression is regulated in response to salt or water stress.
15. The method as claimed in claim 12 , wherein said gene of interest expression is root specific under conditions of water stress.
16. The method as claimed in claim 12 , wherein said transformation is carried out by a method selected from the group consisting of an Agrobacterium -mediated transformation method, and an in-planta transformation method.