Hemagglutinin-binding peptide
Object of the present invention is to provide a hemagglutinin-binding peptide producing an anti-influenza virus effect higher than that of existing peptides. The present invention provides, for example, a hemagglutinin-binding peptide comprising a polypeptide having any of the following amino acid sequences (i) to (iv): (i) Thr-MeGly-Asp-MePhe-MePhe-Ser-MeSer-His-Tyr-Thr-Val-Pro-Arg (SEQ ID NO: 1); (ii) Arg-Val-Ser-MePhe-Thr-Tyr-MePhe-MeSer-Tyr-Thr-Pro-Ser (SEQ ID NO: 2); (iii) an amino acid sequence with deletions, additions, or substitutions of one or several amino acids in SEQ ID NO: 1 or 2; and (iv) an amino acid sequence having 90% or more sequence identity to that of SEQ ID NO: 1 or 2.
1. A peptide comprising an amino acid sequence having 90% or more sequence identity to SEQ ID NO: 1.
2. The peptide according to claim 1 , wherein the peptide has an amino acid deletion, addition, or substitution at position 3, 6, 12, or 13 of SEQ ID NO: 1.
3. The peptide according to claim 1 , wherein the peptide is
(SEQ ID NO: 1)
Thr-MeGly-Asp-MePhe-MePhe-Ser-MeSer-His-Tyr-Thr-
Val-Pro-Arg
(SEQ ID NO: 36)
Thr-MeGly-Lys-MePhe-MePhe-Ser-MeSer-His-Tyr-Thr-
Val-Pro-Arg;
(SEQ ID NO: 37)
Thr-MeGly-Asp-MePhe-MePhe-Ala-MeSer-His-Tyr-Thr-
Val-Pro-Arg;
(SEQ ID NO: 38)
Thr-MeGly-Asp-MePhe-MePhe-Lys-MeSer-His-Tyr-Thr-
Val-Pro-Arg;
(SEQ ID NO: 39)
Thr-MeGly-Asp-MePhe-MePhe-Glu-MeSer-His-Tyr-Thr-
Val-Pro-Arg;
(SEQ ID NO: 40)
Thr-MeGly-Asp-MePhe-MePhe-Ser-MeSer-His-Tyr-Thr-
Val-Hyp-Arg;
(SEQ ID NO: 41)
Thr-MeGly-Asp-MePhe-MePhe-Ser-MeSer-His-Tyr-Thr-
Val-Pro-Ala;
(SEQ ID NO: 42)
Thr-MeGly-Asp-MePhe-MePhe-Ser-MeSer-His-Tyr-Thr-
Val-Pro-Glu;
(SEQ ID NO: 43)
Thr-MeGly-Asp-MePhe-MePhe-Ser-MeSer-His-Tyr-Thr-
Val-Pro-Lys;
or
(SEQ ID NO: 44)
Thr-MeGly-Asp-MePhe-MePhe-Ser-MeSer-His-Tyr-Thr-
Val-Pro-Dap.
4. The peptide according to claim 1 , wherein said peptide is cyclized.
5. The peptide according to claim 4 , wherein said peptide comprises a chloroacetylated amino acid within 3 amino acids from the N-terminus; and cysteine within 3 amino acids from the C-terminus.
6. The peptide according to claim 4 , wherein said peptide comprises chloroacetyl-Trp at the N-terminus, having Cys at the C-terminus, and is cyclized via a thioether bond therebetween.
7. A pharmaceutical composition comprising the peptide according to claim 1 .
8. The peptide according to claim 3 , wherein said peptide is cyclized.
9. The peptide according to claim 8 , wherein said peptide comprises a chloroacetylated amino acid within 3 amino acids from the N-terminus; and cysteine within 3 amino acids from the C-terminus.
10. The peptide according to claim 8 , wherein said peptide comprises chloroacetyl-Trp at the N-terminus, having Cys at the C-terminus, and is cyclized via a thioether bond therebetween.
11. A pharmaceutical composition comprising the peptide according to claim 3 .