Methods and compositions for controlling corn rootworm
The present disclosure provides compositions comprising Corn Rootworm (CRW)-active Methylobacterium sp., methods for controlling CRW, and methods of making the compositions. Also provided are isolated CRW-active Methylobacterium sp.
1. A method for reducing corn rootworm (CRW) mediated root damage to a corn plant, wherein said method comprises: (i) applying a composition comprising CRW-active Methylobacterium sp. ISO04 deposited as NRRL B-50932 to a corn plant or a part thereof at a vegetative emergence (VE), vegetative 1 (V1), vegetative 2 (V2), vegetative 3 (V3) or vegetative 4 (V4) stage of development, or to a corn seed; and, (ii) growing the corn plant or a corn plant from the corn seed in the presence of CRW, thereby reducing CRW mediated root damage to the corn plant or to the corn plant grown from the corn seed, in comparison to a control corn plant grown in the presence of the CRW but without applying as in step (i) the composition comprising the CRW-active Methylobacterium sp. to the control plant.
2. The method of claim 1 , wherein the composition comprises a solid substance with adherent CRW-active Methylobacterium grown thereon or an emulsion having CRW-active Methylobacterium grown therein.
3. The method of claim 2 , wherein the composition comprises the CRW-active Methylobacterium sp. at a titer of about 5×10 8 colony-forming units per gram of the solid substance to about 5×10 13 colony-forming units of Methylobacterium per gram of the solid substance or at a titer of about 1×10 6 CFU/mL, to about 1×10 9 CFU/mL for the emulsion.
4. The method of claim 1 , wherein the composition further comprises an insecticide that provides for inhibition of CRW growth and/or reductions in CRW-mediated plant damage.
5. The method of claim 4 , wherein the insecticide is selected from the group consisting of a pyrethrin, synthetic pyrethroid, oxadiazine, chloronicotinyl, neonicotinoid, nitroguanidine insecticide, triazole, organophosphate, pyrrol, pyrazole, diacylhydrazine, biological/fermentation product, and a carbamate.
6. The method of claim 1 , wherein the applied composition coats or partially coats the corn plant, the part thereof, or the corn seed.
7. The method of claim 1 , wherein the composition is applied to foliage of the corn plant.
8. The method of claim 7 , wherein the corn plant is at about a vegetative 3 (V3) or vegetative 4 (V4)-stage.
9. The method of claim 1 , wherein the composition is applied to the corn seed.
10. The method of claim 1 , wherein the composition is applied to a corn seed comprising a transgene that expresses a gene product that can confer resistance to corn rootworm.
11. The method of claim 1 , wherein the composition is applied to a corn seed comprising a transgene that expresses a gene product that can confer tolerance to a herbicide.
12. The method of claim 1 , wherein the composition is applied to a corn seed comprising a transgene that expresses a gene product that can confer resistance to insects.
13. The method of claim 1 , wherein the composition further comprises a microbial biopesticide.
14. The method of claim 1 , wherein the composition further comprises a seed treatment selected from the group consisting of an insecticide, a fungicide, a nematocide, and a bacteriocide.
15. The method of claim 14 , wherein the seed treatment is selected from the group consisting of pyrethrins, synthetic pyrethroids, oxadiazines, chloronicotinyls, neonicotinoids, nitroguanidine insecticides, triazoles, organophosphates, pyrrols, pyrazoles, diacylhydrazines, and carbamates.
16. The method of claim 14 , wherein the seed treatment is clothianidin.
17. The method of claim 1 , wherein the composition further comprises CRW-active Methylobacterium sp. ISO02 (NRRL B-50930).
18. The method of claim 1 further comprising increasing yield of the corn plant grown in the presence of CRW or yield of the corn plant grown from said seed in the presence of CRW in comparison to yield of the control corn plant.
19. A method for controlling corn rootworm (CRW) damage to a corn plant that comprises: (i) applying a composition comprising a CRW-active Methylobacterium sp. to soil where a corn plant is growing or will be grown, wherein the composition is applied during the growing season, wherein the CRW-active Methylobacterium sp. is ISO04 deposited as NRRL B-50932; and,
(ii) growing a corn plant or a corn plant from corn seed in soil subjected to the application of the composition and in the presence of CRW, wherein CRW damage sustained by the corn plant grown in the presence of the CRW is reduced in comparison to a control plant grown in the presence of the CRW, wherein the control corn plant is grown without applying as in step (i) the composition comprising the CM-active Methylobacterium sp.
20. The method of claim 19 , wherein the composition comprises a solid substance with adherent CRW-active Methylobacterium grown thereon or an emulsion having CRW-active Methylobacterium grown therein.
21. The method of claim 20 , wherein the composition comprises the CRW-active Methylobacterium sp. at a titer of about 5×10 8 , 1×10 9 , or 1×10 10 colony-forming units per gram of the solid substance to about 5×10 13 colony-forming units of Methylobacterium per gram of the solid substance or at a titer of about 1×10 6 CFU/mL to about 1×10 9 CFU/mL for the emulsion.
22. The method of claim 19 , wherein the composition further comprises an insecticide that provides for inhibition of CRW growth and/or reductions in CRW-mediated plant damage.
23. The method of claim 19 , wherein the insecticide is selected from the group consisting of a pyrethrin, synthetic pyrethroid, oxadiazine, chloronicotinyl, neonicotinoid, nitroguanidine insecticide, triazole, organophosphate, pyrrol, pyrazole, diacylhydrazine, biological/fermentation product, and a carbamate.
24. The method of claim 19 , wherein the composition is applied to the soil by broadcasting the composition, by drenching the soil with the composition, and/or by depositing the composition in furrow.
25. The method of claim 24 , wherein the depositing in furrow is performed prior to placing corn seed in the furrow, at the same time as placing corn seed in the furrow, or after placing corn seed in the furrow.
26. The method of claim 19 further comprising increasing yield of the corn plant grown in the presence of CRW or yield of the corn plant grown from said seed in the presence of CRW in comparison to yield of the control corn plant.