Pharmaceutical composition for treatment and/or prevention of hepatitis C
The present invention can induce stronger cellular immunity to hepatitis C and provide a treatment means and a prevention means that are effective in completely eliminating the hepatitis C virus (HCV). Provided is a pharmaceutical composition for the treatment and/or prevention of hepatitis C, said composition comprising a recombinant vaccinia virus (a) and a recombinant vector (b) and characterized in that after one of the recombinant vaccinia virus (a) and the recombinant vector (b) is administered for initial immunity, the other is administered for additional immunity. The recombinant vaccinia virus (a) contains an expression promoter and all or a portion of the cDNA of the HCV genome. The recombinant vector (b) contains an expression promoter and all or a portion of the cDNA of the HCV (where the cDNA contained in the recombinant vector (b) has a different base sequence than that included in the recombinant vaccinia virus (a)).
1. A pharmaceutical composition for treating and/or preventing hepatitis C, comprising a (a) recombinant vaccinia virus and a (b) recombinant vector below:
(a) a recombinant vaccinia virus comprising an expression promoter and DNA of (i) or (ii) below:
(i) DNA comprising the nucleotide sequence represented by SEQ ID NO:1; or
(ii) DNA that comprises a nucleotide sequence having 90% or more identity to DNA comprising the nucleotide sequence represented by SEQ ID NO:1 and that codes for a structural protein and a non-structural protein of hepatitis C virus; and
(b) a recombinant vector comprising an expression promoter and DNA of (iii) or (iv) below:
(iii) DNA comprising the nucleotide sequence represented by SEQ ID NO:2; or
(iv) DNA that comprises a nucleotide sequence having 90% or more identity to DNA comprising the nucleotide sequence represented by SEQ ID NO:2 and that codes for a structural protein and a non-structural protein of hepatitis C virus,
wherein either one of the (a) recombinant vaccinia virus or the (b) recombinant vector is administered for a primary immunization, and then the other is administered for a boost immunization.
2. A pharmaceutical composition for treating and/or preventing hepatitis C, comprising a (a) recombinant vaccinia virus and a (b) recombinant vaccinia virus below:
(a) a recombinant vaccinia virus comprising an expression promoter and DNA of (i) or (ii) below:
(i) DNA comprising the nucleotide sequence represented by SEQ ID NO:1; or
(ii) DNA that comprises a nucleotide sequence having 90% or more identity to DNA comprising the nucleotide sequence represented by SEQ ID NO:1 and that codes for a structural protein and a non-structural protein of hepatitis C virus; and
(b) a recombinant vaccinia virus comprising an expression promoter and DNA of (iii) or (iv) below:
(iii) DNA comprising the nucleotide sequence represented by SEQ ID NO:2; or
(iv) DNA that comprises a nucleotide sequence having 90% or more identity to DNA comprising the nucleotide sequence represented by SEQ ID NO:2 and that codes for a structural protein and a non-structural protein of hepatitis C virus,
wherein either one of the (a) recombinant vaccinia virus or the (b) recombinant vaccinia virus is administered for a primary immunization, and then the other is administered for a boost immunization.
3. The pharmaceutical composition according to claim 1 , wherein the expression promoter is a hybrid promoter.
4. The pharmaceutical composition according to claim 3 , wherein the nucleotide sequence of the hybrid promoter is DNA of the following (a) or (b):
(a) DNA comprising the nucleotide sequence represented by SEQ ID NO:4; or
(b) DNA that comprises a nucleotide sequence having 90% or more identity to DNA comprising the nucleotide sequence represented by SEQ ID NO:4 and that has promoter activity.
5. The pharmaceutical composition according to claim 1 , wherein the vaccinia virus is strain LC16m8.
6. A method for treating and/or preventing hepatitis C, comprising administrating to a patient in need thereof the pharmaceutical composition according to claim 1 , wherein either one of the (a) recombinant vaccinia virus or the (b) recombinant vector is administrated for a primary immunization, and then the other is adminiatrated for a boost immunization.
7. A pharmaceutical composition for treating and/or preventing hepatitis C, comprising a (a) recombinant vaccinia virus and a (b) recombinant vector below:
(a) a recombinant vaccinia virus comprising an expression promoter and DNA of (i) or (ii) below:
(i) DNA comprising the nucleotide sequence represented by SEQ ID NO:2; or
(ii) DNA that comprises a nucleotide sequence having 90% or more identity to DNA comprising the nucleotide sequence represented by SEQ ID NO:2 and that codes for a structural protein and a non-structural protein of hepatitis C virus; and
(b) a recombinant vector comprising an expression promoter and DNA of (iii) or (iv) below:
(iii) DNA comprising the nucleotide sequence represented by SEQ ID NO:1; or
(iv) DNA that comprises a nucleotide sequence having 90% or more identity to DNA comprising the nucleotide sequence represented by SEQ ID NO:1 and that codes for a structural protein and a non-structural protein of hepatitis C virus,
wherein either one of the (a) recombinant vaccinia virus or the (b) recombinant vector is administered for a primary immunization, and then the other is administered for a boost immunization.