Method for converting food waste and other biological waste into invertebrate feed
Biological waste such as food, organic or other biologically-derived waste is converted into shelf-stable and health-safe invertebrate feed. The method for converting includes pre-treating waste by fragmenting, reducing microbial contaminants, optionally amending with components that optimize fermentation, inoculating with microorganisms and mixing. Fermentation takes place in a bioreactor and produces fermentation leachate and solid fermentate. In the post-treatment steps, the solid fermentate is separated from the fermentation leachate. The solid fermentate is ground, dewatered and milled. The solid fermentate can be used as an invertebrate feed with or without further processing.
1. A method for converting a biological waste to a feed for black solider fly larvae, the method comprising:
pre-treating the biological waste, wherein the pretreating comprises:
fragmenting the waste,
reducing microbial contaminants in the waste,
inoculating the waste with microorganisms, and
mixing the waste;
providing a bioreactor;
performing fermentation of the waste under anaerobic conditions, wherein the fermentation comprises fermenting the waste in the bioreactor to produce a fermentation product comprising fermentation leachate and solid fermentate;
post-treating the fermentation product, wherein the post-treating comprises:
separating solid fermentate from fermentation leachate in the fermentation product,
grinding the solid fermentate,
dewatering the solid fermentate, and/or
milling the solid fermentate,
thereby producing the feed for black solider fly larvae.
2. The method of claim 1 wherein the biological waste is optimal biological waste or low efficiency biological waste.
3. The method of claim 1 wherein the pre-treating comprises amending the waste with one or more fermentation-optimizing agents selected from the group consisting of bran, sugars, water, lignocellulose, sodium chloride, sulfite and limestone.
4. The method of claim 1 comprising fragmenting the waste to a particle size of approximately 1 mm to 50 mm.
5. The method of claim 1 wherein the fermentation of the waste occurs at a temperature of approximately 20-45° C.
6. The method of claim 1 wherein the pH of the fermentation leachate is less than or equal to 5.0 after 24-48 hours.
7. The method of claim 1 wherein the Brix % of the feed is at least approximately 6%.
8. The method of claim 1 wherein the density of microorganisms inoculating the waste is ≥10 5 -10 6 cells per ml.
9. The method of claim 1 wherein the pre-treating comprises amending the waste with fermentation optimizing agents consisting essentially of bran, a molasses or corn syrup solution and a biological inoculate.
10. The method of claim 1 wherein reducing microbial contaminants in the waste comprises inoculating the waste with a consortium of microorganisms selected from the group consisting of bacteria and fungi from the genus Acetobacter, Saccharomyces, Lactobacillus and Clostridium.
11. The method of claim 1 wherein the pH of the fermentation leachate is less than or equal to approximately 4.0 in 3 to 7 days.
12. The method of claim 1 wherein the Brix % of the feed is at least approximately 8%.
13. The method of claim 1 wherein after the fermentation leachate is separated from the solid fermentate, the leachate has a pH of approximately 3.4 and a Brix % of approximately 8%.
14. The method of claim 1 wherein the feed contains approximately 8% water, 12-13% crude protein, 11% fat, 5% ash and 63% carbohydrates.
15. A method for converting a biological waste to shelf-stable invertebrate feed, the method comprising:
pre-treating the biological waste, wherein the pretreating comprises:
fragmenting the waste,
reducing microbial contaminants in the waste,
inoculating the waste with microorganisms, and
mixing the waste;
providing a bioreactor;
performing fermentation of the waste under anaerobic conditions, wherein the fermentation comprises fermenting the waste in the bioreactor to produce a fermentation product comprising fermentation leachate and solid fermentate,
wherein the pH of the fermentation leachate is less than or equal to approximately 4 in 3 to 7 days;
post-treating the fermentation product, wherein the post-treating comprises:
separating solid fermentate from fermentation leachate in the fermentation product,
grinding the solid fermentate,
dewatering the solid fermentate, and/or
milling the solid fermentate,
thereby producing the invertebrate feed wherein the invertebrate feed has a stable pH of approximately 3.4.
16. The method of claim 15 wherein the fermentation does not produce, within approximately ≥0.1-1 ppm level of change, methane, hydrogen sulfide, ammonia, nitrous oxide and volatile chemicals associated with putrefaction.
17. The method of claim 15 wherein the invertebrate feed is stable and acidic at room temperature for at least 12 months when kept anaerobic.
18. A method for converting a biological waste to an invertebrate feed, the method comprising:
(a) pre-treating the biological waste, wherein the pretreating comprises:
fragmenting the waste,
reducing microbial contaminants in the waste,
inoculating the waste with non-pathogenic mesophilic microorganisms at a density of 10 5 -10 6 cells per ml, wherein the microorganisms are:
chemo-organotrophs,
aerotolerant anaerobes or facultative anaerobes,
acidophilic or acid tolerant at pH >3.2,
cellulolithic,
amylolytic, [or]
homofermenters or heterofermenters individually and heterofermenters as a community;
wherein the microorganisms have the capacity to:
ferment pentoses and hexoses, thereby producing alcohols and organic acids,
drive fermentation toward acidic conditions,
produce low levels of soluble alkali,
hydrolyze cellulose and starch to monosaccharides or disaccharides of hexoses or pentoses, and
wherein the microorganisms comprise microorganisms from the genera Acetobacter, Lactobacillus, Saccharomyces , and Clostridium , and
mixing the waste;
(b) providing a bioreactor;
(c) performing fermentation of the waste under anaerobic conditions in the bioreactor, thereby producing a fermentation product comprising fermentation leachate having a Brix % of 6-10 and solid fermentate, wherein performing fermentation comprises circulating fermentation leachate in the bioreactor during fermentation,
(d) monitoring the fermentation, wherein monitoring comprises analyzing the fermentation leachate,
wherein analyzing the fermentation leachate comprises determining pH, fermentable sugar content and nitrogen content of the fermentation leachate,
(e) controlling progression of the fermentation, wherein controlling progression of the fermentation comprises maintaining:
temperature at 20-45° C.,
pH <5,
Brix % at 9-10 Brix % during the first 24 hours of the fermentation, and
Brix % at least 3 Brix % after 24 hours of the fermentation,
(f) post-treating the fermentation product, wherein the post-treating comprises:
separating solid fermentate from fermentation leachate in the fermentation product,
grinding the solid fermentate,
dewatering the solid fermentate, and/or
milling the solid fermentate,
thereby producing the invertebrate feed.
19. The method of claim 18 wherein the biological waste is optimal biological waste or low efficiency biological waste.
20. The method of claim 18 wherein the pre-treating comprises amending the waste with one or more fermentation-optimizing agents selected from the group consisting of bran, sugars, water, lignocellulose, sodium chloride, sulfite and limestone.
21. The method of claim 18 comprising fragmenting the waste to a particle size of approximately 1 mm to 50 mm.