Spot array substrate, method for producing same, and nucleic acid polymer analysis method and device
In order to reduce the cost of producing a spot array substrate and reduce the cost of nucleic acid polymer analysis, a spot array substrate is used which is produced by preparing a resin substrate 402 having a surface on which an uneven pattern is formed and a plurality of bead sitting positions set in a two-dimensional array within the uneven pattern, and loading surface-modified beads onto the bead sitting positions of the resin substrate.
1. A spot array substrate comprising:
a resin substrate having a surface including an uneven pattern that includes a plurality of grooves that form rhombic patterns, and a plurality of bead sitting positions set in a two-dimensional array within the uneven pattern; and
a plurality of surface-modified beads loaded into the bead sitting positions of the resin substrate,
wherein a thermal expansion coefficient of a material of the resin substrate is in a range from 10 −5 to 10 −4 /° C.,
wherein the plurality of bead sitting positions are intersections of the plurality of grooves disposed in the resin substrate,
wherein an epoxy group is disposed as a film only on inner surfaces of the respective plurality of grooves of the resin substrate,
wherein the respective surface-modified beads loaded in the bead sitting positions are physically clamped by inner surfaces of the grooves of the bead sitting positions, which are physically contracted, and
wherein the surface-modified beads are at least one of functional group surface-modified beads and oligo DNA surface-modified beads.
2. The spot array substrate according to claim 1 , wherein the grooves have a smaller width than the dimensions of the bead sitting positions.
3. The spot array substrate according to claim 1 , wherein the respective surface-modified beads are immobilized at the respective bead sitting positions of the resin substrate by chemical bonding.
4. The spot array substrate according to claim 1 , wherein the bulk material of the surface-modified beads is a material including silicon oxide or a material including a magnetic body.
5. The spot array substrate according to claim 1 , wherein the surface-modified beads are functional group surface-modified beads.
6. The spot array substrate according to claim 1 , wherein the surface-modified beads are oligo DNA surface-modified beads.
7. The spot array substrate according to claim 1 , wherein the resin substrate contains a cycloolefin polymer or a cycloolefin copolymer as a component.
8. The spot array substrate according to claim 1 , wherein the surface density of the bead sitting positions is 6.6×10 6 /cm 2 to 180×10 6 /cm 2 .
9. The spot array substrate according to claim 1 , wherein a size of the respective surface-modified beads is equal to a size of the respective intersections of the grooves.