Enzyme-linked immunosorbent assay (ELISA) for the detection of anti-mycoplasma hyorhinis IgG in swine serum
This disclosure presents an Enzyme-Linked Immunosorbent Assay (ELISA) for the selective detection of anti- Mycoplasma hyorhinis IgG in porcine serum, which may contain antibodies specific to multiple other Mycoplasma spp.
1. A purified, recombinant, non-naturally occurring, non-post-translationally modified, Mycoplasma hyorhinis ( M. hyorhinis ) p37 polypeptide having a sequence with at least 98% identity to the sequence as set forth in SEQ ID NO:5; wherein the polypeptide is useful for the selective detection of M. hyorhinis antibodies.
2. The polypeptide of claim 1 , wherein the polypeptide has the sequence as set forth in SEQ ID NO:5.
3. An Enzyme-Linked Immunosorbent Assay kit comprising the polypeptide of claim 1 .
4. A method for making an ELISA plate, for use as a component of the kit of claim 3 , comprising the following steps:
a) suspending the polypeptide of claim 1 in carbonate/bicarbonate buffer to a concentration of about 80 μg/mL to about 120 μg/mL, to produce a coating solution;
b) adding from about 80 μL to about 120 μL of the coating solution to each well;
c) incubating the plate for at least about 12 hours at about 4° C.;
d) removing the coating solution;
e) blocking the plate;
f) washing the plate; thereby preparing the ELISA assay plate.