Injectable, flexible hydroxyapatite-silk foams for osteochondral and dental repair
The present invention provides, among other things, a silk ceramic material having enzymatically cross-linked amino acid side chains to generate injectable and flexible foam ceramics. Provided are compositions and methods of producing soft, flexible ceramic foam with silk polymeric crosslinking to serve as binders. Materials have applications in osteochondral and dental replacement and repair.
1. A method of producing a silk ceramic material, the method comprising steps of: subjecting a composition comprising:
silk fibroin; and
a calcium phosphate material;
to enzymatic crosslinking under conditions and for a period of time sufficient to convert the material from a first state that is substantially free of beta sheet structure with no solid form structure to a second solidified state having more than 70% beta sheet structure.
2. The method of claim 1 wherein the silk fibroin is present in the composition at a weight percent within the range of about 0.1% to about 50%, inclusive.
3. The method of claim 1 wherein the calcium phosphate material is present in the composition at a weight percent within the range of about 30% to about 50%.
4. The method of claim 1 , wherein the calcium phosphate material and silk fibroin are present in relative weight percent amounts within the range of 1:2 to 4:5.
5. The method of claim 1 , wherein the silk fibroin has a molecular weight within the range of about 10 kDa to about 450 kDa.
6. The method of claim 1 , wherein the composition is a solution.
7. The method of claim 1 , wherein the composition is a paste.
8. The method of claim 1 , wherein:
the composition further comprises a crosslinking enzyme; and
the step of subjecting comprises adding to the composition a substrate for the enzyme so that the enzyme introduces crosslinks into the composition.
9. The method of claim 8 , wherein the crosslinks comprise crosslinks between silk fibroin tyrosine residues.
10. The method of claim 8 , wherein the crosslinking enzyme is selected from a peroxidase, a lignin peroxidase, laccase, tyrosinase, an oxidase, and an oxidoreductase.
11. The method of claim 1 , wherein the second state is characterized as having at least one of a compressive toughness of between 1-20 kJm 3 , inclusive or a compressive elastic modulus between 1-5 MPa at 5% strain, inclusive.
12. The method of claim 1 , further comprising a step of providing the composition by combining:
a solution comprising the silk fibroin; and
a powder comprising the calcium phosphate material.
13. The method of claim 12 , wherein the solution is prepared by a process that includes boiling for a period of time within the range of 1 minute to 120 minutes, inclusive.
14. The method of claim 12 , wherein the boiling is performed under conditions and for a period of time so that the silk fibroin has a molecular weight within the range of 300 kDa to 450 kDa.
15. The method of claim 12 , wherein the boiling is performed under conditions and for a period of time so that the silk fibroin has a molecular weight within the range of 10 kDa to 300 kDa.
16. The method of claim 12 , wherein the step of providing the composition comprises combining the powder and the solution in a relative proportion within the range of 0.5 to 0.8.