ANALYSIS METHOD AND ANALYSIS DEVICE
Provided is a high-accuracy analysis method utilizing an enzyme-lined immunoassay. The presence of an analyte 3 can be detected or the abundance of the analyte 3 can be analyzed by: bonding an antibody 5 that is capable of specifically bonding to the analyte 3 immobilized on a solid phase 1 and has an enzyme 7 bonded thereto; then decomposing an enzyme substrate 8, which can generate decomposition products capable of being detected easily with a mass spectrometry, with the enzyme 7 bonded to the antibody 5; and then analyzing the decomposition products 9 and 10 with a mass spectrometry.
1 . An analysis method for measuring an analyte,
wherein an analyte is immobilized on a solid phase,
wherein an antibody to which a label to be specifically bonded to the analyte immobilized on a-the solid phase is bonded to the analyte,
wherein a label bonded substance having galactosidase bonded thereto is bonded to the label,
wherein enzyme reaction is caused to occur between the galactosidase bonded to the label bonded substance and an enzyme substrate, and
wherein mass spectrometry is performed on an enzyme reaction product of the obtained enzyme substrate so as to measure the presence or absence and concentration of the analyte.
2 . (canceled)
3 . The analysis method according to claim 1 ,
wherein as the label and the label bonded substance, biotin and avidin are used.
4 . The analysis method according to claim 1 , comprising:
a step of immobilizing the analyte on the solid phase;
a step of bonding the antibody to be specifically bonded to the analyte and having the galactosidase bonded thereto to the analyte;
an enzyme reaction step of adding the enzyme substrate and causing the enzyme substrate to react with the galactosidase for a prescribed time; and
an analysis step of causing a mass spectrometer to analyze the obtained enzyme reaction product.
5 .- 6 . (Canceled)
7 . The analysis method according to claim 1 ,
wherein the enzyme reaction product serving as an analysis target is a compound in which logP serving as a hydrophobicity index is 1 to 5 and a molecular weight is 150 to 1,000.
8 . The analysis method according to claim 7 ,
wherein the enzyme reaction product has a structure in which a plurality of aromatic compounds are linked by a linker including single bonding of C—N or C—O, and is a compound having the molecular weight of 200 to 600.
9 . An analysis device for measuring an analyte, the device comprising:
an enzyme reaction unit that immobilizes an analyte on a solid phase, that bonds an antibody to which a label to be specifically bonded to the analyte immobilized on the solid phase is bonded, to the analyte, that bonds a label bonded substance having galactosidase bonded thereto to the label, and that so as to cause enzyme reaction to occur between the galactosidase bonded to the label bonded substance and an enzyme substrate; and
a mass spectrometry unit that performs mass spectrometry on an enzyme reaction product of the obtained enzyme substrate,
wherein the analysis device measures the presence or absence and concentration of the analyte.
10 . (canceled)
11 . The analysis device according to claim 9 ,
wherein as the label and the label bonded substance, biotin and avidin are used.
12 . The analysis device according to claim 9 ,
wherein the enzyme reaction unit immobilizes the analyte on the solid phase, bonds the antibody to be specifically bonded to the analyte and having the galactosidase bonded thereto to the analyte, adds the enzyme substrate, and causes the enzyme substrate to react with the galactosidase for a prescribed time so as to generate the enzyme reaction product, and
wherein the mass spectrometry unit causes a quadrupole mass spectrometer to analyze the obtained enzyme reaction product.
13 . (canceled)
14 . The analysis device according to claim 9 ,
wherein the enzyme reaction product serving as an analysis target is a compound in which logP serving as a hydrophobicity index is 1 to 5 and a molecular weight is 150 to 1,000.
15 . The analysis device according to claim 14 ,
wherein the enzyme reaction product has a structure in which a plurality of aromatic compounds are linked by a linker including single bonding of C—N or C—O, and is a compound having the molecular weight of 200 to 600.