Optical analyzing device
This optical analyzing device is provided with a light source, a detector, a substrate having a metal film on at least one surface thereof, and an optical element for introducing a light beam from the light source to the substrate and delivering the light beam from the substrate toward the detector. A plurality of sample regions for holding samples are provided on the metal film; and a portion of the light beam from the light source is irradiated to any one of the sample regions, is reflected, at least once, by the surface of the substrate on the opposite side of the side on which the sample regions are provided, and is not irradiated to a sample region other than the aforementioned sample region in the path thereof until the portion of the light beam is delivered by the optical element.
1. An optical analyzing device comprising:
a light source;
a detector;
a substrate that has a metal film on at least one surface thereof; and
one optical element that introduces a light beam to the substrate from the light source through a first portion of the one optical element, and that guides the light beam from the substrate to the detector after the light beam exits the substrate through a second portion of the one optical element,
wherein a plurality of sample regions for holding samples are disposed on the metal film,
wherein a portion of the light beam from the light source is emitted to any of the sample regions, is reflected twice or more on the metal film, is reflected at least once on a surface opposite to a side having the sample regions in the substrate, and is not emitted to other sample regions excluding the sample regions in an optical path until the portion of the light beam is guided by the optical element, and
wherein a recess is disposed on a surface of the one optical element between the first portion of the one optical element and the second portion of the one optical element.
2. The optical analyzing device according to claim 1 , further comprising:
a plurality of reagent holding units for holding a reagent; and
a plurality of valves for switching whether to feed the reagent to the sample regions from any of the reagent holding units.
3. The optical analyzing device according to claim 1 ,
wherein a substance having a known reflectance is disposed between sample regions adjacent to each other.
4. The optical analyzing device according to claim 1 , further comprising:
a gas cylinder that introduces CO2 to the substrate.
5. The optical analyzing device according to claim 1 , further comprising:
a channel component that is connected to the substrate, and that has a groove for forming a channel on the substrate; or
a well component that forms a well for holding a liquid.
6. The optical analyzing device according to claim 1 , further comprising:
a holder that fixes the substrate, a channel component, and the optical element to each other.
7. The optical analyzing device according to claim 1 ,
wherein the substrate and the optical element have an integrated structure.
8. The optical analyzing device according to claim 1 ,
wherein an index-matching liquid is disposed between the substrate and the optical element.
9. The optical analyzing device according to claim 1 ,
wherein the substrate and the optical element are formed of the same material.
10. The optical analyzing device according to claim 1 ,
wherein the optical element is a prism.
11. The optical analyzing device according to claim 1 ,
wherein images in the detector which correspond to the respective sample regions do not overlap each other.
12. The optical analyzing device according to claim 1 ,
wherein a light beam width (D/cos Θ) of the light from the light source is narrower than a width from one end to another end of the sample region, and is wider than the sum of widths of the sample regions which are concurrently measured.
13. The optical analyzing device according to claim 1 ,
wherein a light beam width (D/cos Θ) of the light from the light source is narrower than a width of the reflecting region, and is wider than the sum of widths of the sample regions which are concurrently measured.
14. The optical analyzing device according to claim 12 ,
wherein the light from the light source is concurrently emitted to a region including all of the sample regions at least at one point of time.
15. An optical analyzing device comprising:
a light source;
a detector;
a substrate that has a metal film on at least one surface thereof; and
one optical element that introduces a light beam to the substrate from the light source through a first portion of the one optical element, and that guides the light beam from the substrate to the detector side after the light beam exits the substrate through a second portion of the one optical element,
wherein a plurality of sample regions for holding samples are disposed on the metal film, and
wherein the substrate includes a reflecting member disposed on a surface opposite to a side having the sample regions,
wherein a portion of the light beam from the light source is emitted to any of the sample regions, is reflected twice or more on the metal film, and is reflected at least once on the reflecting member, and
wherein a recess is disposed on a surface of the one optical element between the first portion of the one optical element and the second portion of the one optical element.