Cell culture method and cell culture apparatus
A bottom surface of a container ( 1 ) formed of a flexible material is partially raised to be partitioned it into plural compartments ( 10 ), and cells are cultured in each compartment ( 10 ). In due time, the compartments ( 10 ) are removed to expand a culture area in the container. As a result, the cell density at the time of culture can be maintained at an appropriate level, and an operation of transferring cells from one culture container to another culture container at the time of proliferating cells in a large amount can be eliminated, whereby damage on cells and risk of contamination can be reduced.
1. A method of culturing cells comprising:
supplying a culture medium together with cells to a container formed of a flexible material, and
conducting cell culture in the container, wherein,
the container is held on a base having a flat mounting surface and in which partition pieces that can be protruded from the mounting surface are embedded
a bottom surface of the container is partially raised by partition pieces protruding from the mounting surface, thereby to partition the bottom surface into a plurality of compartments due to flexural deformation of the flexible material,
the cells are cultured in each compartment where flat parts excluding deforming parts that contact the mounting surface serve as a culture surface; and
thereafter, the deforming parts are flattened, the compartments are removed to expand a culture area in the container, and culture of cells is continued.
2. The method of culturing cells according to claim 1 , wherein the partition pieces are accommodated into the base, and upper end surfaces of the partition pieces are made flush with the mounting surface, thereby to remove the compartments.
3. The method of culturing cells according to claim 1 , wherein,
when cell culture is continued by expanding the culture area in the container by removing the compartments,
moving the container to a position higher than an initial position while being inclined by rotatably moving the base pivotably supported at one end thereof by a supporting frame, and then the container is returned to the initial position by rotatably moving the base as if it falls down, and
vibration that is due to an impact generated by receiving of the base by the supporting frame is applied to the container that has returned to the initial position thereby to stir a content liquid in the container.
4. A method of culturing cells comprising:
supplying a culture medium together with cells to a container formed of a flexible material, and
conducting cell culture in the container, wherein,
the container is held on a base having a flat mounting surface, in which partition pieces that can be protruded from the mounting surface are embedded, and to which a pressing plate is attached such that the pressing plate can be moved up and down,
a bottom surface of the container is partially raised by partition pieces protruding from the mounting surface, thereby to partition the bottom surface into a plurality of compartments due to flexural deformation of the flexible material,
while pressing the container by the pressing plate fixed at a first position, the cells are cultured in each compartment where flat parts excluding deforming parts that contact the mounting surface serve as a culture surface, whereby cell culture is conducted with the inside of the container being pressurized, and
thereafter, when the deforming parts are flattened, the compartments are removed to expand a culture area in the container, and cell culture is continued, fixing the pressing plate at a second position that is higher than the first position, and
supplying additional culture medium to the container such that an amount of a content thereof is increased by supplying the additional culture medium is pressed by the pressing plate, whereby the cell culture is continued with the inside of the container being pressurized so that flow of the content liquid that damages the cells is suppressed.