Cell membrane-derived nanovesicles and use thereof
Disclosed are cell membrane-derived nanovesicles, a method of preparing the nanovesicles, and a pharmaceutical composition and a diagnostic kit using the nanovesicles. The cell membrane-derived nanovesicles may prevent potential adverse effects because intracellular materials (e.g., genetic materials and cytosolic proteins) unnecessary for delivering therapeutic or diagnostic substances are removed from the nanovesicles. In addition, as the nanovesicles may be targeted to specific cells or tissues, therapeutic or diagnostic substances may be predominantly delivered to the targeted cells or tissues, while delivery of the substances may be inhibited. Therefore, the nanovesicles may alleviate suffering and inconvenience of patients by reducing adverse effects of therapeutic substances and by improving efficacy of the substances. In addition, the cell membrane-derived nanovesicles loaded with therapeutic or diagnostic substances and a method of preparing the nanovesicles may be used in vitro or in vivo for therapeutic or diagnostic purposes, or for experimental use.
1. Nanovesicles prepared from membranes of cells,
wherein intracellular components including genetic materials and cytosolic proteins are removed in the nanovesicles by treating the cells with an alkaline solution of pH 9 to 14 and sonication of the treated cells, and the size of the nanovesicles is smaller than the size of the cells,
wherein the nanovesicles are loaded with one or more substances for treating or diagnosing a disease selected from the group consisting of an anti-cancer agent, an anti-inflammatory agent, an angiogenesis inhibitor, a toxin, a bead, a microparticle, a nanoparticle, and a fluorescence-emitting material, and
wherein the nanovesicles retain topology of membrane proteins of the cells from which the nanovesicles originate.
2. The nanovesicles according to claim 1 , wherein the cells are capable of targeting specific types of cells or tissues.
3. The nanovesicles according to claim 1 , wherein the cells are genetically transformed to express one or more selected from the group consisting of cell adhesion molecules, antibodies, targeting proteins, cell membrane fusion proteins, and fusion proteins thereof.
4. The nanovesicles according to claim 1 , wherein the cells are genetically transformed to express one or more selected from the group consisting of growth factors, cytokines, receptors, fluorescent proteins, and fusion proteins thereof.
5. The nanovesicles according to claim 1 , wherein membranes of the nanovesicles further comprises non-cellular membrane components,
wherein the noncellular membrane components are selected from the group consisting of cyclodextrin, polyethylene glycol, and chemically modified membrane components thereof.