Patent
The present invention relates to extracts derived from plants and/or fractions thereof, compositions comprising same and use thereof in protecting tissues from damages caused by exogenous toxins. In particular, the present invention relates to polar extract and/or fraction thereof derived from Asteriscus graveolens , compositions comprising same and use thereof in protecting tissues comprising epithelial cell from the deleterious effect of exogenous toxins, particularly toxins originating from environmental pollution.
1. A method for protecting a tissue comprising epithelial cells from the deleterious effect of at least one pollutant comprising:
topically administering to a skin of a subject in need thereof a composition comprising an effective dose of a polar solvent extract derived from Asteriscus graveolens plant, a fraction thereof or any combination of a polar solvent extract derived from Asteriscus graveolens plant and a fraction thereof, so as to result in protecting the tissue from the at least one pollutant, wherein the at least one pollutant originates from at least one of combustion gas, industrial pollution and smoke.
2. The method of claim 1 , wherein the effective dose is from 0.1% to 10% w/w relative to the total weight of the composition.
3. The method of claim 1 , wherein the smoke is tobacco smoke.
4. The method of claim 1 , wherein a test, to determine whether the composition results in protecting the tissue from the at least one pollutant comprises
culturing epithelial cells in different concentrations of cigarette smoke-intoxicated medium containing the composition; and
measuring epithelial cell viability in the different concentrations of cigarette smoke-intoxicated medium containing the composition,
wherein there is an increase in IC 50 ,
wherein the increase represents an increase in the dose of the cigarette smoke-intoxicated medium necessary to have a significant toxic effect, representing a protective effect of the composition.
5. The method of claim 1 , wherein protecting the skin from the deleterious effect of the at least one pollutant results in at least one outcome selected from the group consisting of increased skin cell viability, improved skin appearance, reduced premature skin aging and any combination thereof.
6. The method of claim 5 , wherein improved skin appearance and/or reduced premature skin aging comprises reduction in the appearance of at least one of wrinkles, brown spots and red spots of the skin.
7. The method of claim 1 , wherein the polar solvent is water.
8. The method of claim 1 , wherein the polar solvent comprises water and at least one additional polar solvent.
9. The method of claim 8 , wherein the at least one additional polar solvent is selected from the group consisting of glycerin, ethanol, propylene glycol, butylene glycol, methanol, and acetone.
10. The method of claim 9 , wherein the polar solvent comprises water and glycerin.
11. The method of claim 10 , wherein the polar solvent extract and/or fraction thereof is in a form of a solution comprising 40 to 60% (w/w) glycerin.
12. The method of claim 1 , wherein the polar solvent extract or a fraction thereof is essentially devoid of essential oils.
13. The method of claim 1 , wherein the composition further comprises an additional active agent selected from the group consisting of an anti-oxidant, a chelator, a cleansing agent, a skin protectant, a sunscreen, a skin lightening agent, an anti-wrinkling agent, an anti-inflammatory agent, an anti-aging agent, and any combination thereof.
14. The method of claim 1 , wherein the composition is a cosmetic composition formulated for topical administration to the skin.