Method for quantification of virus particles using capillary zone electrophoresis
The present invention provides a method for the quantification of virus particles in a biological sample, comprising the steps of: (a) introducing said biological sample comprising virus particles into a capillary tube containing a buffer solution; (b) applying an electrical field to said capillary tube of sufficient voltage to allow for the separation of the virus particles from additional constituents in said sample, to obtain electrophoretical fractions; (c) generating an electropherogram associated with the electrophoretical fractions; and (d) determining the concentration of virus particles in said sample by comparing the electropherogram with an electropherogram generated from a reference sample containing a known concentration of said virus particles.
1. A method for direct quantification of adenovirus particles in a biological sample, comprising:
a) introducing the biological sample comprising virus particles into a capillary tube containing a buffer solution;
b) applying an electrical field to the capillary tube of 10,000 to 30,000 Volts to allow for the separation of the adenovirus particles from additional constituents in the sample, to obtain electrophoretical fractions;
c) generating an electropherogram associated with the electrophoretical fractions; and
d) determining the concentration of the adenovirus particles in the sample by comparing the electropherogram with an electropherogram generated from a reference sample containing a known concentration of the adenovirus particles,
wherein the buffer solution comprises tris(hydroxymethyl)aminomethane in a concentration of between about 100 and 500 mM and tricine in a concentration of between 100 and 500 mM, and has a pH of between 7.3 and 8.9, and wherein the capillary tube is flushed in reverse direction with a phosphoric acid solution before the capillary tube is flushed and filed with the buffer solution.
2. The method according to claim 1 , wherein the buffer solution comprises tris(hydroxymethyl)aminomethane in a concentration of between about 100 and 300 mM and tricine in a concentration of between 100 and 412.5 mM, and has a pH of between 7.5 and 8.5.
3. The method according to claim 1 , wherein the buffer comprises tris(hydroxymethyl)aminomethane in a concentration of between about 100 and 150 mM, and tricine in a concentration of between 300 and 350 mM and has a pH of between about 7.5 and 7.8.
4. The method according to claim 1 , wherein the buffer solution further comprises polysorbate 20 in a concentration of between 0.1 and 0.3% v/v.
5. The method according to claim 1 , wherein the capillary tube is a neutral coated capillary tube.
6. The method according to claim 5 , wherein the capillary tube is coated with a polyvinyl alcohol (PVA) or a fluorocarbon polymer.
7. The method according to claim 1 , wherein the phosphoric acid solution comprises 5 to 20 mM phosphoric acid.
8. The method according to claim 1 , wherein the separation is performed at a temperature of about 15° C.
9. The method according to claim 1 , wherein the adenovirus particles are recombinant adenovirus particles.
10. The method according to claim 1 , wherein the adenovirus particles are adenovirus 5, adenovirus 26 or adenovirus 35 particles.
11. A method for direct quantification of adenovirus particles in a sample, comprising:
a) introducing the sample into a neutral coated capillary tube containing a buffer solution;
b) applying an electrical field to the neutral coated capillary tube at a voltage of 10,000 to 30,000 Volts to allow for the separation of the adenovirus particles from additional constituents in the sample, to thereby obtain electrophoretical fractions;
c) generating an electropherogram associated with the electrophoretical fractions; and
d) determining the concentration of the adenovirus particles in the sample by comparing the electropherogram with an electropherogram generated from a reference sample containing a known concentration of the adenovirus particles,
wherein,
the buffer solution comprises 100-150 mM tris(hydroxymethyl)aminomethane, 300-350 mM tricine and 0.1-0.3% polysorbate, and has a pH of 7.5 to 7.8, and
the capillary tube is flushed, in a direction from outlet to inlet of the capillary tube, with a phosphoric acid solution before the capillary tube is filed with the buffer solution.
12. The method according to claim 11 , wherein the neutral coated capillary tube is coated with a polyvinyl alcohol (PVA).
13. The method according to claim 11 , wherein the neutral coated capillary tube is coated with a fluorocarbon polymer.
14. The method according to claim 11 , wherein the phosphoric acid solution comprises 5 to 20 mM phosphoric acid.
15. The method according to claim 11 , wherein the separation is performed at a temperature of about 15° C.
16. The method according to claim 11 , wherein the electrical field is applied to the neutral coated capillary tube at a voltage of 25,000 Volts.
17. The method according to claim 11 , wherein the buffer solution comprises 125 mM tris(hydroxymethyl)aminomethane, 338 mM tricine and 0.2% polysorbate 20.