Ophthalmic compositions and methods of use therefor
The present invention encompasses ophthalmic compositions that may be used for various conditions of the eye, and particularly, conditions of the cornea. Also encompassed are methods that utilise these compositions and kits that include these compositions.
1. A method of: (i) treating or preventing progression of a condition associated with a thinning or irregularity of a cornea; or (ii) treating or preventing progression of a refractive error of an eye, the method comprising:
contacting the cornea or the eye with a composition comprising a TGFβ3 polypeptide which comprises the amino acid sequence of SEQ ID NO: 1 or which is at least 90% identical to the amino acid sequence of SEQ ID NO: 1, and dexamethasone or derivative thereof or a related steroidal agent, wherein the contacting with the composition does not result in scarring in the cornea or in the eye,
thereby treating or preventing progression of the condition of the cornea or the refractive error of the eye.
2. The method of claim 1 , wherein:
(a) the TGFβ3 polypeptide consists of the amino acid sequence of SEQ ID NO: 1; and/or
(b) the dexamethasone is dexamethasone phosphate.
3. The method of claim 1 , wherein:
(a) the composition comprises 10 to 100 ng/ml of the TGFβ3 polypeptide; and/or
(b) the composition comprises 40 to 4000 ng/ml dexamethasone.
4. The method of claim 1 , wherein:
(a) the composition is administered once daily or twice daily;
(b) the composition is co-administered with one or more additional agents for the eye; and/or
(c) the composition is administered in conjunction with use of a contact lens, corneal insert, corneal implant, or intrastromal ring.
5. The method of claim 4 , wherein:
(a) the one or more additional agents for the eye are selected from the group consisting of: anaesthetic agents, anti-inflammatory agents, anti-microbial agents, and lubricants;
(b) the contact lens, corneal insert, corneal implant, or intrastromal ring is adapted for moulding or holding corneal shape during and/or following treatment with the composition; and/or
(c) the contact lens, corneal insert, corneal implant, or intrastromal ring is adapted to act as a carrier for the composition or as a composition eluting device.
6. The method of claim 1 , wherein:
(a) for (i), the composition is administered prior to and/or subsequent to corneal collagen crosslinking; or
(b) for (ii), the method is performed preceding or following refractive surgery.
7. The method of claim 1 , wherein:
(a) the condition is selected from the group consisting of: keratoconus, myopia, and astigmatism;
(b) the refractive error of the eye is associated with one or more of: myopia, hyperopia, astigmatism, and presbyopia.
8. A method of inducing collagen type II expression in a keratocyte, the keratocyte being in an eye having (i) a condition associated with a thinning or irregularity of a cornea; or (ii) a refractive error, and the method comprising:
contacting the keratocyte with a composition comprising a TGFβ3 polypeptide which comprises the amino acid sequence of SEQ ID NO:1 or which is at least 90% identical to the amino acid sequence of SEQ ID NO:1, and dexamethasone or derivative thereof or a related steroidal agent, wherein the contacting with the composition does not result in scarring in the eye,
thereby inducing collagen type II expression in the keratocyte.
9. The method of claim 8 , wherein:
(a) the TGFβ3 polypeptide consists of the amino acid sequence of SEQ ID NO:1; and/or
(b) the dexamethasone is dexamethasone phosphate.
10. The method of claim 8 , wherein:
(a) the composition comprises 10 to 100 ng/ml of the TGFβ3 polypeptide; and/or
(b) the composition comprises 40 to 4000 ng/ml dexamethasone.
11. The method of claim 8 , wherein:
(a) the composition is formulated for administration via a contact lens, a corneal insert, a corneal implant, or an intrastromal ring;
(b) the composition is formulated for administration as a solution, gel, cream, or emulsion; and/or
(c) the composition is formulated for administration in vivo or ex vivo.
12. A method of: (i) treating or preventing progression of a condition associated with a thinning or irregularity of a cornea; or (ii) treating or preventing progression of a refractive error of an eye, the method comprising:
co-administering to the cornea or to the eye a composition comprising a TGFβ3 polypeptide which comprises the amino acid sequence of SEQ ID NO: 1 or which is at least 90% identical to the amino acid sequence of SEQ ID NO: 1, and a composition comprising dexamethasone or derivative thereof or a related steroidal agent, wherein co-administration does not result in scarring in the cornea or in the eye,
thereby treating or preventing progression of the condition of the cornea or the refractive error of the eye.
13. A method of inducing collagen type II expression in a keratocyte, the keratocyte being in an eye having (i) a condition associated with a thinning or irregularity of a cornea; or (ii) a refractive error, and the method comprising:
co-administering to the keratocyte a composition comprising a TGFβ3 polypeptide which comprises the amino acid sequence of SEQ ID NO:1 or which is at least 90% identical to the amino acid sequence of SEQ ID NO:1, and a composition comprising dexamethasone or derivative thereof or a related steroidal agent, wherein co-administration does not result in scarring in the cornea or in the eye,
thereby inducing collagen type II expression in the keratocyte.