Porcine epidemic diarrhea virus strains and immunogenic compositions therefrom
The present invention is directed to novel nucleotide and amino acid sequences of Porcine Epidemic Diarrhea Virus (“PEDV”), including novel genotypes thereof, all of which are useful in the preparation of vaccines for treating and preventing diseases in swine and other animals. Vaccines provided according to the practice of the invention are effective against multiple swine PEDV genotypes and isolates. Diagnostic and therapeutic polyclonal and monoclonal antibodies are also a feature of the present invention, as are infectious clones useful in the propagation of the virus and in the preparation of vaccines. Particularly important aspects of the invention include polynucleotide constructs that replicate in tissue culture and in host swine. The invention also provides for novel full length PEDV genomes that can replicate efficiently in host animals and tissue culture.
1. An isolated Porcine Epidemic Diarrhea Virus (PEDV) encoded by a DNA polynucleotide sequence that is SEQ ID NO: 36 or SEQ ID NO: 37.
2. A vaccine composition comprising a porcine epidemic diarrhea virus (PEDV) according to claim 1 , and a carrier, wherein said composition is capable of protecting swine from challenge by both variant and prototype strains of PEDV and preventing or treating one or more symptoms associated with PEDV infection, and wherein achievement of protection is determined by an endpoint selected from the group consisting of prevention or control of any of the PEDV infection symptoms of dehydration, fever, diarrhea, vomiting, poor lactational performance, poor reproduction performance, mortality, and prevention or control of weight loss or failure to gain weight.
3. The vaccine composition of claim 2 wherein the virus is live or killed.
4. The vaccine composition of claim 2 wherein said carrier is a diluent.
5. The vaccine composition of claim 2 further comprising an adjuvant.
6. The vaccine composition of claim 2 wherein said protected swine include any of sows, gilts, boars, hogs, and piglets.
7. The vaccine composition of claim 5 wherein the adjuvant is de-oiled lecithin dissolved in an oil and aluminum hydroxide.
8. The vaccine composition of claim 5 , wherein said adjuvant is CpG/DEAE-dextran/mineral oil (TXO).
9. A method of preventing disease in a piglet caused by PEDV, said method comprising administering to said piglet the vaccine composition of claim 2 , wherein a first dose of said vaccine is administered when the piglet is about 1-7 days old.
10. The method of claim 9 , wherein said administering further comprises administering a second dose of said vaccine when the piglet is about 2-5 weeks old.
11. The method of claim 9 , wherein 2 doses are administered to the piglet, and the parent sow, although vaccinated pre-breeding, was not vaccinated pre-farrowing.
12. The method of claim 9 , wherein 2 doses are administered to the piglet, and the parent sow is vaccinated pre-farrowing.
13. The method of claim 9 , wherein only a single dose is administered to the piglet, and wherein the mother sow of the piglet is naïve to PEDV, and is not, at any time, vaccinated.
14. An isolated Porcine Epidemic Diarrhea Virus (PEDV) that is encoded by a DNA polynucleotide sequence that is at least 95% or 99% identical, at a full length nucleotide level, to SEQ ID: NO:37, wherein said nucleotide sequence comprises one or both of (1) an ORF1a/1b protein having a proline residue at the amino acid position thereof corresponding to position 551 in SEQ ID NO:46; and (2) a spike protein having a histidine residue at the amino acid position thereof corresponding to position 973 in SEQ ID NO: 47.
15. A full length RNA polynucleotide that corresponds to the encoding DNA polynucleotide of claim 1 , or the complement thereof.
16. The RNA polynucleotide of claim 15 that is an infectious clone.
17. A plasmid or bacterial artificial chromosome that comprises the encoding DNA polynucleotide of claim 1 .