Light amount detection device, immune analyzing apparatus and charged particle beam apparatus that each use the light amount detection device
A signal detected by a photomultiplier tube is pre-amplified and converted into a digital signal. A time average value of signal components, each of which has a voltage lower than a predetermined base threshold value, is calculated as a base voltage. A signal that has been subjected to base correction processing is subjected to threshold value processing and to base correction processing in a non-incident state in which light is not incident on the photomultiplier tube. An output signal thereof is subjected to dark current calculation processing; and a light emission signal amount is calculated by subtracting, from the signal component of the detection light obtained by the threshold value processing, a time average value of the signal components of the dark current. As the result, discriminating the dark current pulse from floor noises enhances the accuracy of the base voltage, and thus the accuracy of light detection.
1. A light amount detection device comprising:
an amplifier that performs amplification processing of amplifying a detection signal from a light detection unit for detecting light;
an A/D converter that performs A/D conversion processing of converting the detection signal amplified by the amplifier into a digital signal to output the digital signal;
a base voltage calculation part that performs base voltage calculation processing of, for the output signal from the A/D converter, calculating, as a base voltage, a time average value of signal components each having a voltage lower than a predetermined base threshold value;
a base correction processing part that performs base correction processing of offsetting the output signal from the A/D converter in such a manner that the base voltage calculated by the base voltage calculation part becomes 0;
a dark current calculation part that performs dark current calculation processing of, for an output signal from the base voltage calculation processing part in a non-input state in which light is not input into the light detection unit, calculating, as a dark current pulse, a signal component having a voltage higher than a predetermined signal detection threshold value;
a threshold value processing part that performs threshold value processing of, for the output signal from the base voltage calculation processing part, calculating, as a detection light pulse, a signal component having a voltage higher than a predetermined signal detection threshold value; and
a light emission signal amount calculation part that performs light emission signal amount calculation processing of calculating a light emission signal amount by subtracting, from the signal component of the detection light obtained by the threshold value processing, a time average value of the signal components of the dark current obtained by the dark current calculation processing.
2. The light amount detection device according to claim 1 ,
wherein the base voltage calculation processing and the dark current calculation processing are executed in parallel.
3. The light amount detection device according to claim 1 , further comprising
an output signal storage unit that stores an output signal from the A/D converter, and outputs the output signal as necessary,
wherein the dark current calculation processing, the threshold value processing, and the light emission signal amount calculation processing are performed on the basis of the output signal from the output signal storage unit.
4. The light amount detection device according to claim 1 , further comprising
a detection signal switching unit that switches between presence and absence of an output of a detection signal from the light detection unit.
5. The light amount detection device according to claim 1 ,
wherein the base voltage calculation processing is performed in a non-input state in which light is not input into the light detection unit, or in a state in which no detection signal is output from the light detection unit.
6. An immune analyzing apparatus comprising:
a flow cell into which a mixed solution is introduced, the mixed solution being composed of a sample to be measured, and a reagent containing a light-emitting element and magnetic particles, the reagent being combined with a specific component in the sample to form a complex;
a magnetic field generator that generates a magnetic field for capturing the complex in the mixed solution, which has been introduced into the flow cell, in a predetermined measurement region in the flow cell; and
the light amount detection device according to claim 1 , the light amount detection device detecting light generated from the complex captured in the measurement region.
7. A charged particle beam apparatus comprising:
a sample stage on which a sample to be measured is arranged;
a charged particle beam irradiation device that irradiates the sample on the sample stage with a charged particle beam; and
the light amount detection device according to claim 1 , the light amount detection device detecting light generated in a scintillator by a secondary electron that is obtained by irradiating the sample with the charged particle beam.