IP Library Granted Patent US 11,702,620
Granted Patent B2
US 11,702,620 · App. 15/566,427 · Granted Jul 18, 2023

Self-contained anaerobic environment-generating culture device

Inventors: Evan D. Brutinel (Inver Grove Heights, MN); Mary B. Forester (Eagan, MN); Adam J. Stanenas (Cottage Grove, MN); Jason W. Bjork (Cottage Grove, MN)
Assignee: 3M Innovative Properties Company
C12M1/045C12M1/005C12M1/007C12M1/14C12M23/14C12M23/22C12M25/02C12M25/06C12M41/34C12M41/36C12N1/20C12Q1/04
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Quick Facts
Patent No.
US 11,702,620
App. No.
15/566,427
Granted
Jul 18, 2023
Kind
B2
Abstract

A device for culturing anaerobic microorganisms is provided. The device comprises a body comprising a waterproof base, a waterproof coversheet attached to the base, and a growth compartment disposed between the base and the coversheet. The growth compartment has a perimeter and an opening that provides liquid access to the growth compartment. A portion of the perimeter is defined by a waterproof seal. The portion includes >50% of the perimeter. A dry cold water-soluble gelling agent is adhered to the base in the growth compartment. A dry first oxygen-scavenging reagent is disposed in the growth compartment.

Claims (25)

1. A device, comprising:

a body comprising a waterproof base, a waterproof coversheet attached to the base, and a growth compartment disposed therebetween, the growth compartment having a perimeter and an opening that provides liquid access to the growth compartment;

wherein a portion of the perimeter is defined by a waterproof seal, wherein the portion includes >50% of the perimeter;

a dry cold water-soluble gelling agent adhered to the base in the growth compartment; and

a dry first oxygen-scavenging reagent disposed in the growth compartment.

2. The culture device of claim 1 , further comprising a dry carbon dioxide-generating reagent disposed in the growth compartment.

3. The culture device of claim 2 , wherein the carbon dioxide-generating reagent consists essentially of particles having a diameter of 100 microns or less.

4. The device of claim 1 , further comprising a dry reducing agent disposed in the growth compartment.

5. The device of claim 1 , further comprising a dry nutrient, wherein the nutrient is disposed in the growth compartment, wherein the nutrient facilitates growth of an anaerobic microorganism, wherein the nutrient is not the oxygen-scavenging reagent.

6. The device of claim 5 , wherein the nutrient comprises a nonfermentable organic carbon source.

7. The device of claim 1 , further comprising a dry second oxygen-scavenging reagent disposed in the growth compartment.

8. The device of claim 1 , further comprising an indicator reagent for detecting a presence of a viable microorganism, wherein the indicator reagent is disposed in the growth compartment.

9. The device of claim 1 , wherein a first dry component selected from the group consisting of a first oxygen-scavenging reagent, a second oxygen-scavenging reagent, a reducing reagent, a nutrient, a indicator reagent and a combination of any two or more of the foregoing components is adhered to the base.

10. The device of claim 9 , wherein the base comprises a first adhesive layer disposed thereon in at least a portion of the growth compartment, wherein the first dry component is adhered to the first adhesive layer in the growth compartment.

11. The device of claim 9 , further comprising a first carrier, wherein the first dry component is adhered to the first carrier, wherein the first carrier is adhered to the first adhesive layer in the growth compartment.

12. The device of claim 9 , wherein a second dry component selected from the group consisting of the first oxygen-scavenging reagent, the second oxygen-scavenging reagent, the reducing reagent, the nutrient, the indicator reagent and a combination of any two or more of the foregoing components is adhered to the coversheet.

13. The device of claim 12 :

wherein the coversheet comprises a third adhesive layer disposed thereon in at least a portion of the growth compartment;

wherein the second dry component is adhered to the third adhesive layer in the growth compartment.

14. The device of claim 12 , further comprising a second carrier, wherein the second dry component is adhered to a second carrier, wherein the second carrier is adhered to the second adhesive layer in the growth compartment.

15. The device of claim 1 , wherein the first planar waterproof substrate further comprises a first removable tab proximate the opening.

16. The device of claim 15 , wherein the first removable tab comprises a first closure adhesive adhered thereto.

17. The device of claim 1 , wherein the coversheet further comprises a second removable tab proximate the opening.

18. The device of claim 17 , wherein the second removable tab comprises a second closure adhesive adhered thereto.

19. The device of claim 16 , further comprising a first release liner releasably adhered to the first closure adhesive.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 18, 2024
From: 3M INNOVATIVE PROPERTIES COMPANY,
To: NEOGEN FOOD SAFETY US HOLDCO CORPORATION
Reel/Frame 069736/0835 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 13, 2017
From: BRUTINEL, EVAN D.; FORESTER, MARY B.; STANENAS, ADAM J.; BJORK, JASON W.
To: 3M INNOVATIVE PROPERTIES COMPANY
Reel/Frame 043860/0703 →
Continuity (2)
Provisional Application 62154684 · Apr 29, 2015
Related Publication 20180100131A1 · Apr 12, 2018