Portable nucleic acid extraction apparatus and method of using the same
A portable nucleic acid extraction kit includes a portable nucleic acid extraction apparatus, at least one lysis buffer, at least one binding buffer, at least one washing buffer, at least one elution buffer, and at least three kit sample tubes. The portable nucleic acid extraction apparatus includes a handle, a rod, a tip, and may include a nucleic acid binding medium. The method of using the portable nucleic acid extraction apparatus includes, treating a biological sample having a nucleic acid, binding the nucleic acid in the treated biological sample to the tip of the portable nucleic acid extraction apparatus, incubating the tip of the portable nucleic acid extraction apparatus bound with the nucleic acid, washing the tip of the portable nucleic acid apparatus bound with the nucleic acid, eluting the bound nucleic acid, and analyzing the eluted nucleic acid.
1. A portable nucleic acid extraction apparatus for nucleic acid extraction, comprising:
a handle having a distal end, where the handle is cylindrical in shape;
a rod having a proximal end, where the rod is cylindrical in shape having a diameter from 2-4 millimeters and a length of 1 to 20 centimeters, wherein the proximal end of the rod is attached to the distal end of the handle; and
a tip attached to the rod, where an end of the tip opposite to the rod is closed where an interior of the tip cannot contact nucleic acids, where the tip comprises a material chosen from at least one of polystyrene, functionalized polystyrene, and a silica-derived material,
wherein the portable nucleic acid extraction apparatus does not comprise specialized equipment selected from the group consisting of pipettes, centrifuges, and automated equipment; and
wherein the portable nucleic acid extraction apparatus does not comprise a nucleic acid binding medium that is stationary and not attached to the portable nucleic acid extraction apparatus.
2. The portable nucleic acid extraction apparatus of claim 1 , wherein
the handle, the rod, and the tip are formed as a single piece, where the tip has a cylindrical shape, where the material that forms the handle, the rod, and the tip is selected from at least one of the group consisting of polystyrene, functionalized polystyrene, and a silica-derived material.
3. The portable nucleic acid extraction apparatus of claim 2 , wherein
the handle, the rod, and the tip are silica-derived material, and wherein the tip is sandblasted.
4. The portable nucleic acid extraction apparatus of claim 2 , wherein
the handle, the rod, and the tip are glass sandblasted to increased surface area.
5. The portable nucleic acid extraction apparatus of claim 1 , wherein
the tip is spherical in shape and is configured to bind nucleic acids.
6. The portable nucleic acid extraction apparatus of claim 1 , wherein
the tip is glass sandblasted to increased surface area.
7. A portable nucleic acid extraction apparatus for nucleic acid extraction, comprising:
a handle having a distal end;
a means for allowing portability of the portable nucleic acid extraction apparatus between a first sample tube and a second sample tube, and between a second sample tube and a third sample tube, the means attached to the distal end of the handle; and
a tip attached to the means, where an end of the tip opposite to the means is closed where an interior of the tip cannot contact nucleic acids, where the tip comprises a material chosen from at least one of polystyrene, functionalized polystyrene, and a silica-derived material, wherein the portable nucleic acid extraction apparatus does not comprise specialized equipment selected from the group consisting of pipettes, centrifuges, and automated equipment; and
wherein the portable nucleic acid extraction apparatus does not comprise a nucleic acid binding medium that is stationary and not attached to the portable nucleic acid extraction apparatus.
8. The nucleic acid extraction apparatus of claim 1 , wherein the material of the tip is configured to bind nucleic acids in an acidic buffer having a pH below 6.0.
9. The nucleic acid extraction apparatus of claim 1 , wherein the material of the tip is configured to bind nucleic acids in an acidic buffer having a pH below 6.0 and at least one salt.
10. The nucleic acid extraction apparatus of claim 7 , wherein The material of the tip is configured to bind nucleic acids in an acidic buffer having a pH below 6.0.
11. the nucleic acid extraction apparatus of claim 7 , wherein The material of the tip is configured to bind nucleic acids in an acidic buffer having a pH below 6.0 and at least one salt.