METHOD FOR HYBRIDIZING A NUCLEIC ACID MOLECULE

1 . A method for hybridizing a nucleic acid molecule, comprising

i) providing a reaction mixture comprising

at least one first nucleic acid molecule comprising at least partially hybridized against each other

a sense nucleic acid strand having

at least a first nucleotide sequence, and

at least a second nucleotide sequence;

an antisense nucleic acid strand having at least a third nucleotide sequence being at least partially complementary to said at least second nucleotide sequence and being at least partially non-complementary to said at least first nucleotide sequence; and

at least one second nucleic acid molecule having a fourth nucleotide sequence being at least partially complementary to said first nucleotide sequence; and

hybridization buffer; and

ii) incubating said reaction mixture under conditions allowing said at least one first nucleic acid molecule to hybridize to said at least one second nucleic acid molecule via a hybridization reaction between said at least first nucleotide sequence and said at least fourth nucleotide sequence.

2 . The method of claim 1 , wherein said at least one first nucleic acid molecule is a circular nucleic acid molecule.

3 . The method of claim 1 , wherein said at least one second nucleic acid molecule is linked to a solid support.

4 . The method of claim 1 , wherein said at least one first nucleic acid molecule is provided as follows:

providing said sense nucleic acid strand,

synthesizing said antisense nucleic acid strand, and

hybridizing said sense nucleic acid strand to said antisense nucleic acid strand.

5 . The method of claim 1 , wherein said at least one first nucleic acid molecule is provided as follows:

providing said sense nucleic acid strand,

hybridizing at least one primer oligonucleotide to at least a first segment of said second nucleotide sequence, said at least first segment is located upstream and/or downstream of said first nucleotide sequence, and

extending said primer oligonucleotide by a nucleotide polymerase to generate said antisense nucleic acid strand.

6 . The method of claim 5 , wherein before and/or during said extension a blocking oligonucleotide is hybridized to at least a second segment of said second nucleotide sequence, said second segment is located downstream of said first nucleotide sequence.

7 . The method of claim 1 , wherein said at least first nucleic acid molecule is provided as follows:

providing a double-stranded nucleic acid molecule comprising at least partially hybridized against each other

said sense nucleic acid strand and said antisense nucleic acid strand hybridized against each other, and

an interposed antisense strand, said interposed antisense strand is at least partially hybridized to said first nucleotide sequence, and

degradation of said interposed antisense strand by a nuclease.

8 . The method of claim 1 , wherein said fourth nucleotide sequence is longer than said first nucleotide sequence.

9 . The method of claim 1 , wherein said at least one first nucleic acid molecule and said at least one second nucleic acid molecule are DNA.

10 . The method of claim 1 , wherein said at least one second nucleic acid molecule is a single stranded nucleic acid molecule.

11 . The method of claim 1 , comprising the following further step:

iii) removing said antisense strand,

12 . The method of claim 11 , wherein the removal of said antisense strand is realized by denaturation and/or via a strand displacement by using a polymerase with strand displacement activity.

13 . The method of claim 3 , wherein said solid support comprises a material selected from the group consisting of: metal, glass, silica, and plastics.

14 . (canceled)

15 . (canceled)

16 . The method of claim 5 , wherein said polymerase exhibits no strand displacement activity and no 5′-3′ exonuclease activity.

17 . The method of claim 3 , wherein said solid support is selected from the group consisting of: chips, beads, and capillaries.

18 . A composition comprising:

at least one first nucleic acid molecule comprising at least partially hybridized against each other

a sense nucleic acid strand having

at least a first nucleotide sequence, and

at least a second nucleotide sequence;

an antisense nucleic acid strand having at least a third nucleotide sequence being at least partially complementary to said at least second nucleotide sequence and being at least partially non-complementary to said at least first nucleotide sequence; and

at least one second nucleic acid molecule having a fourth nucleotide sequence being at least partially complementary to said first nucleotide sequence;

19 . The composition of claim 18 , further comprising hybridization buffer.