Device and process for automated extraction of nucleic acids
A device and method are useful for automated extraction of nucleic acids. The device can include a body that can be immersed partly or completely in a reaction cavity, where the part immersed in the reaction cavity has a non-smooth surface. After lysis, an organic substance, preferably alcohols or ketones, can be mixed with a biological sample. This mixture can be brought into contact with a material with a non-smooth surface. Under these conditions, nucleic acids may be adsorbed on the surface of the material being used. Washing steps may be carried out. After drying, the adsorbed nucleic acid may be detached from the material by adding water or a buffer of low salt concentration, whereupon it can be used for downstream applications.
1. A device for automated extraction of a nucleic acid, the device comprising:
at least one body that can be immersed partly or completely in a reaction cavity containing a nucleic acid, wherein at least a part of the at least one body immersed in the reaction cavity has a rough or structured surface,
wherein the rough or structured surface is configured to bind to the nucleic acid during automated extraction of the nucleic acid and when the rough or structured surface is immersed in the reaction cavity, and
wherein the at least one body comprises a smooth hollow body on which a rough or structured object is mounted externally, the rough or structured object being a ring or a sleeve mounted externally on the smooth hollow body.
2. The device according to claim 1 , wherein
the rough or structured object mounted externally on the smooth hollow body of the device is a rough or structured polymer material, a composite material with rough or structured surface or a material produced by 3D printing.
3. The device according to claim 1 , wherein
the hollow body is configured to receive a liquid disposed in the reaction cavity.
4. An instrument for automated extraction of nucleic acid, the instrument comprising:
at least one device according to claim 1 .
5. The instrument according to claim 4 ,
wherein the instrument is an automated pipetting system or an automated extraction system.
6. A method for automated extraction of a nucleic acid, the method comprising:
providing a nucleic acid in a reaction cavity;
immersing the device according to claim 1 in the reaction cavity; and
binding the nucleic acid on the rough or structured surface of the device.