Method for microbial antigen collection
This invention provides a method for easily collecting antigens possessed by microorganisms without the use of special equipment. The method for collecting microbial antigens comprises: allowing a specimen containing microorganisms to pass through a filter membrane with a pore diameter that does not allow microorganisms to pass therethrough; capturing the microorganisms in the specimen on the filter membrane; applying a microbial destruction reagent capable of microbial membrane destruction to the filter membrane comprising the microorganisms captured thereon to destruct the captured microorganisms on the filter membrane; and collecting antigens in the filtrate.
1. A method for collecting antigens of microorganisms comprising: allowing specimens containing microorganisms to pass through a filter membrane with a pore diameter that does not allow the microorganisms to pass therethrough; capturing the microorganisms in the specimens on the filter membrane; allowing a microbial destruction reagent capable of microbial membrane destruction to pass through the filter membrane on which the microorganisms are captured to destruct the captured microorganisms on the filter membrane; and collecting the antigens in the filtrate, wherein the microbial destruction reagent is a surfactant, an alkaline solution, or a mixture of a surfactant and an alkaline solution.
2. The method for collecting antigens of microorganisms according to claim 1 , wherein the microorganisms are bacteria.
3. The method for collecting antigens of microorganisms according to claim 1 , wherein the microbial destruction reagent is a mixture of a surfactant and an alkaline solution.
4. The method for collecting antigens of microorganisms according to claim 3 , wherein the alkaline solution has a pH of 11 or higher.
5. The method for collecting antigens of microorganisms according to claim 3 , wherein the alkaline solution is a sodium hydroxide solution.
6. A method for detecting microorganisms comprising assaying the microbial antigens collected by the method according to claim 1 by an immunoassay technique.
7. The method for detecting microorganisms according to claim 6 , wherein the immunoassay technique is immunochromatography.
8. A method for detecting microorganisms comprising assaying the microbial antigens collected by the method according to claim 2 by an immunoassay technique.
9. A method for detecting microorganisms comprising assaying the microbial antigens collected by the method according to claim 3 by an immunoassay technique.
10. A method for detecting microorganisms comprising assaying the microbial antigens collected by the method according to claim 4 by an immunoassay technique.
11. A method for detecting microorganisms comprising assaying the microbial antigens collected by the method according to claim 5 by an immunoassay technique.
12. The method for detecting microorganisms according to claim 8 , wherein the immunoassay technique is immunochromatography.
13. The method for detecting microorganisms according to claim 9 , wherein the immunoassay technique is immunochromatography.
14. The method for detecting microorganisms according to claim 10 , wherein the immunoassay technique is immunochromatography.
15. The method for detecting microorganisms according to claim 11 , wherein the immunoassay technique is immunochromatography.