Immunochromatographic device reduced in background noise and method for reduced background noise
An object of the present invention is to improve visibility by reducing background noise in order to accurately detect the signal from a target substance in an immunochromatographic device. The immunochromatographic device comprises a membrane having a detection region, to which an antibody or antigen serving as a capturing substance capable of capturing a target substance is immobilized, for detecting the target substance by using an antigen or antibody labeled with a labeling carrier that is a colored particle to form a complex of the capturing substance-target substance-labeled antigen or antibody in the capturing substance-immobilized detection region on the device, based on color of the labeling carrier, wherein a colorant having a color complementary to the color of the labeling carrier is allowed to be contained in a dry state in a constituent member of the device such that the colorant is developed together with a specimen when the specimen is developed on the device.
1. An immunochromatographic device comprising
a membrane having a detection region, to which an antibody or antigen serving as a capturing substance capable of capturing a target substance is immobilized, for detecting the target substance by using an antigen or antibody labeled with a labeling carrier that is a colored particle to form a complex of the capturing substance-target substance-labeled antigen or antibody in the capturing substance-immobilized detection region on the device, based on color of the labeling carrier,
wherein a colorant having a color complementary to the color of the labeling carrier is allowed to be contained in a dry state in a sample pad arranged on an upstream side of the detection device such that the colorant is developed together with a specimen when the specimen is developed on the device.
2. The immunochromatographic device according to claim 1 , wherein the colorant is a dye or a pigment.
3. The immunochromatographic device according to claim 1 , wherein the colorant is a color particle.
4. A method for reducing background noise in an immunochromatographic device, the device comprising
a membrane having a detection region, to which an antibody or antigen serving as a capturing substance capable of capturing a target substance is immobilized, for detecting the target substance by using an antigen or antibody labeled with a labeling carrier that is a colored particle to form a complex of the capturing substance-target substance-labeled antigen or antibody in the capturing substance-immobilized detection region on the device, based on color of the labeling carrier,
the method comprising
allowing a colorant having a color complementary to the color of the labeling carrier to be contained in a dry state in a sample pad arranged on an upstream side of the detection region of the device; and
allowing the colorant to develop together with a specimen on the device when a specimen is developed on the device, thereby reducing the intensity of color of the background noise derived from the labeling carrier.
5. The method for reducing background noise according to claim 4 , wherein the colorant is a dye or a colorant.
6. The method for reducing background noise according to claim 4 , wherein the colorant is a color particle.