Acetate consuming yeast cell
The present invention relates to a yeast cell that is genetically modified comprising: a) a disruption of one or more aldehyde dehydrogenase (E.C:1.2.1.4) native to the yeast; b) one or more nucleotide sequence encoding a heterologous NAD + -dependent acetylating acetaldehyde dehydrogenase (E.C. 1.2.1.10); c) one or more nucleotide sequence encoding a homologous or heterologous acetyl-CoA synthetase (E.C. 6.2.1.1); and d) a modification that leads to reduction of glycerol 3-phosphate phosphohydrolase (E.C. 3.1.3.21) and/or glycerol 3-phosphate dehydrogenase (E.C. 1.1.1.8 or E.C. 1.1.5.3) activity, native to the yeast.
1. A yeast cell that is genetically modified comprising:
a) a disruption of one or more nucleotide sequence encoding an aldehyde dehydrogenase-6 (ALD6) (E.C. 1.2.1.4) native to the yeast;
b) one or more nucleotide sequence encoding a heterologous NAD+-dependent acetylating acetaldehyde dehydrogenase (E.C. 1.2.1.10);
c) one or more nucleotide sequence encoding a homologous or heterologous acetyl-CoA synthetase (E.C. 6.2.1.1); and
d) a disruption or mutation in one or more endogenous gene encoding glycerol 3-phosphate phosphohydrolase (E.C. 3.1.3.21) and/or glycerol 3-phosphate dehydrogenase (E.C. 1.1.1.8 or E.C. 1.1.5.3), whereby said disruption or mutation reduces the amount of glycerol 3-phosphate phosphohydrolase and/or glycerol 3-phosphate dehydrogenase activity;
e) one or more nucleotide sequence encoding a heterologous glycerol dehydrogenase (E.C. 1.1.1.6);
f) one or more nucleotide sequence encoding a homologous or heterologous dihydroxyacetone kinase (E.C. 2.7.1.28 or E.C. 2.7.1.29); and
wherein the yeast cell is a pentose and glucose fermenting yeast cell that is capable of anaerobic simultaneous pentose and glucose consumption.
2. The yeast cell according to claim 1 , wherein the yeast cell is a Saccharomyces cerevisiae , and wherein the one or more aldehyde dehydrogenase (E.C. 1.2.1.4) native to the yeast in a) is a Saccharomyces cerevisiae acetaldehyde dehydrogenase-6 (ALD6).
3. The yeast cell according to claim 1 , wherein the yeast cell comprises
one or more nucleotide sequence encoding a heterologous xylose isomerase (E.C. 5.3.1.5).
4. The yeast cell according to claim 1 , wherein the yeast cell comprises a reduction in the expression or activity of one or more of the genes glycerol phosphate phosphatase 1 (gpp1), glycerol phosphate phosphatase 2 (gpp2), NAD-dependent glycerol 3-phosphate dehydrogenase 1 (gpd1), and NAD-dependent glycerol 3-phosphate dehydrogenase 2 (gpd2) native to the yeast, as compared to the wild-type yeast.
5. A process comprising fermenting a composition comprising acetate, pentose, and hexose with a yeast cell according to claim 4 , wherein the fermentation time required for substantially complete fermentation of the composition is reduced as compared to the fermentation time required for substantially complete fermentation of the composition by said yeast cell that is not genetically modified according to a), b), c), and d).
6. The process according to claim 5 , wherein the fermentation time is reduced 40% or more.
7. The process according to claim 5 , wherein the fermentation produces ethanol, and the overall ethanol production rate is at least 20% greater than the overall ethanol production rate with the yeast cell that is not genetically modified according to a), b), c), and d).
8. The process according to claim 5 , wherein a hydrolysate of lignocellulosic material is fermented.
9. The process according to claim 8 , wherein the hydrolysate is an enzymatic hydrolysate of lignocellulosic material.
10. A process according to claim 8 , wherein the hydrolysate comprises acetate.
11. A process according to claim 10 , wherein the acetate comprising hydrolysate has an acetate concentration of 5% (w/w) or more.