Immune tolerant and non-immune tolerant elastin-like recombinant peptides and methods of use
Disclosed herein, are recombinant polypeptides comprising one or more homologous amino acid repeats; and, non-immunogenic bioconjugates comprising recombinant polypeptides comprising one or more homologous amino acid repeats and one or more therapeutic agents. Also, disclosed herein are pharmaceutical compositions including the recombinant polypeptides; and methods of administering the recombinant polypeptides to patients for the treatment of cancer or infections.
1. A recombinant polypeptide comprising an homologous amino acid repeat sequence, wherein the homologous amino acid repeat sequence is Gly-Val-Leu-Pro-Gly-Val-Gly (SEQ ID NO: 1: iTEP A ), wherein the homologous amino acid repeat sequence is reiterated linearly.
2. A recombinant polypeptide comprising
(SEQ ID NO: 23)
Gly-(Gly-Val-Leu-Pro-Gly-Val-Gly) 28 -Gly-Gly;
(SEQ ID NO: 24)
Gly-(Gly-Ala-Gly-Val-Pro-Gly) 70 -Gly-Gly;
(SEQ ID NO: 25)
Gly-(Val-Pro-Gly-Phe-Gly-Ala-Gly-Ala-Gly) 21 -Gly-
Gly; or
(SEQ ID NO: 26)
Gly-(Val-Pro-Gly-Leu-Gly-Ala-Gly-Ala-Gly) 96 -Gly-
Gly.
3. The recombinant polypeptide of claim 1 , further comprising two or more of the homologous amino acid repeat sequences reiterated linearly.
4. A pharmaceutical composition comprising the recombinant polypeptide of claim 1 and a pharmaceutically acceptable carrier.
5. A pharmaceutical composition comprising the recombinant polypeptide of claim 2 and a pharmaceutically acceptable carrier.