Microbial system for biosynthesis of natural and engineered products coupled to in situ extraction in supercritical CO

1. A method of producing a bioproduct, comprising culturing a cell in a multiphase reactor comprising an aqueous phase and a solvent phase, wherein the solvent phase comprises supercritical CO 2 (scCO 2 ), near critical CO 2 , or liquid CO 2 , wherein the bioproduct is selected from the group consisting of isobutanol, butanol, isopentanol, phenyl-ethyl-alcohol and 4-methyl-pentanol; and/or wherein the bioproduct is a hydrocarbon, oxygenated hydrocarbon, aldehyde, alcohol, fatty acid, or ketone.

2. The method of claim 1 , wherein

(a) the cell is a viable cell;

(b) the cell is a bacterial cell;

(c) the cell is in spore-form or has been acclimated to scCO 2 , optionally wherein the cell has been acclimated to scCO 2 by previous exposure to scCO 2 ; and/or

(d) the cell is engineered to recombinantly express one or more genes.

3. The method of claim 2 , wherein the bacterial cell is resistant to scCO 2 and/or the bacterial cell is a spore-forming bacterium belonging to the genus Firmicutes , optionally wherein the bacterial cell is a Bacillus spp, optionally selected from the group consisting of Bacillus cereus, Bacillus subterraneus, Bacillus amyloliquefaciens, Bacillus safensis , and Bacillus megaterium , optionally Bacillus megaterium SR7.

4. The method of claim 1 , wherein the bioproduct is partitioned into the scCO 2 , near critical CO 2 , or liquid CO 2 and/or wherein the bioproduct is isolated from the solvent phase and/or the aqueous phase.

5. The method of claim 1 , wherein the bioproduct is isobutanol and the cell is engineered to recombinantly express an isoketovalerate decarboxylase and an alcohol dehydrogenase; optionally, wherein the isoketovalerate decarboxylase is a Lactococcus lactis gene and/or wherein the alcohol dehydrogenase is an E. coli gene.

6. The method of claim 1 , wherein the bioproduct is 4-methyl-pentanol and the cell is engineered to recombinantly express one or more enzymes selected from the group consisting of an acetolactate synthase, a ketol-acid reductoisomerase, a dihydroxy-acid dehydratase, an alpha-ketoisovalerate decarboxylase, an aldehyde dehydrogenase, a propionyl-CoA transferase, a beta-keto-thiolase, a beta-keto-acyl-CoA reductase, an enoyl-CoA hydratase, a trans-enoyl-CoA reductase, a carboxylic acid reductase, a 4′-phosphopantenheinyl transferase, and an alcohol dehydrogenase.

7. The method of claim 1 , wherein the solvent phase further comprises less than or equal to about 3% inert helium, and/or wherein the aqueous phase comprises a growth media, optionally wherein the growth media comprises a spore germination inducer that is optionally D-alanine.

8. The method of claim 1 , wherein the culturing comprises incubating the cell in the multiphase reactor for at least 5 hours and/or the culturing comprises incubating the cell in the multiphase reaction at a temperature between 35° C.-40° C.