Identification of tumor-associated markers for diagnosis and therapy
The present technology relates to genetic products the expression of which is associated with cancer diseases. The present technology also relates to the therapy and diagnosis of diseases in which the genetic products are expressed or aberrantly expressed, in particular cancer diseases.
1. A method of detecting a nucleic acid sequence consisting of SEQ ID NO: 44 in a cancer cell, wherein the method comprises the steps of:
detecting the presence of or determining the quantity of the nucleic acid sequence consisting of SEQ ID NO: 44 expressed in the cancer cell in a biological sample comprising tissue isolated from a patient, wherein
the detecting or determining comprises (i) contacting the biological sample with an agent that binds specifically to the nucleic acid, and (ii) detecting the formation of or determining the quantity of a complex between the agent and the nucleic acid, wherein said agent is an oligonucleotide or polynucleotide that hybridizes specifically to the nucleic acid or to the complementary nucleic acid sequence;
and
wherein said cancer cell is from a cancer or tumor selected from the group consisting of ovarian cancer, colorectal cancer, lung cancer, prostate cancer, melanoma, and cervical cancer.
2. The method of claim 1 , wherein the method comprises detection of the presence of or determination of the quantity of nucleic acid in a first sample at a first point in time and in a further sample at a second point in time.
3. The method of claim 1 , wherein the agent is labeled in a detectable manner.
4. The method of claim 1 , wherein tissue is from a tissue biopsy.
5. The method of claim 1 , wherein the detecting or determining comprises real-time reverse-transcription polymerase chain reaction (RT-PCR).