IP Library Granted Patent US 10,918,627
Granted Patent B2
US 10,918,627 · App. 15/592,090 · Granted Feb 16, 2021

Convergent and enantioselective total synthesis of Communesin analogs

Inventors: Mohammad Movassaghi (Lincoln, MA); Stephen Paul Lathrop (San Mateo, CA); Matthew W. Pompeo (Cambridge, MA); Wen-Tau Timothy Chang (Billerica, MA)
Assignee: Massachusetts Institute of Technology
A61K31/437A61K31/407A61K31/55A61K45/06C07D487/22Y02A50/30
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Quick Facts
Patent No.
US 10,918,627
App. No.
15/592,090
Granted
Feb 16, 2021
Kind
B2
Abstract

A highly convergent biomimetic synthesis of a complex polycyclic scaffold has been successfully implemented. From these efforts, compounds having a structure of Formula (I): or a pharmaceutically acceptable salt, tautomer or stereoisomer thereof, wherein R 1 -R 8 and m, n, r, s, t, and u are as defined herein, is provided. Methods of making such compounds are also disclosed as are methods for the treatment of cancer, various infectious diseases, and abnormal cardiovascular function.

Claims (60)

1. A compound of Formula (I):

or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein:

R 1 and R 4 are each independently selected from H, C 1 -C 12 alkyl, C 2 -C 12 alkenyl, C 2 -C 12 alkynyl, —C(═O)R 9 , —C(═O)NR 9 R 10 , —S(═O) u R 12 , aryl, heteroaryl, carbocyclyl, and heterocyclyl;

R 3 is selected from C 1 -C 12 alkyl, C 2 -C 12 alkenyl, C 2 -C 12 alkynyl, —C(═O)R 9 , —C(═O)NR 9 R 10 , —S(═O) u R 12 , aryl, heteroaryl, carbocyclyl, and heterocyclyl, or R 3 and R 4 taken together with the atoms to which they are attached form a heteroaryl or heterocyclic ring;

R 2 and R 5 are each independently selected from F, Cl, Br, I, —OH, —OR 9 , —OC(═O)R 9 , —S(═O) u R 12 , —NR 9 R 10 , C 1 -C 12 alkyl, C 2 -C 12 alkenyl, C 2 -C 12 alkynyl, aryl, heteroaryl, carbocyclyl, and heterocyclyl;

R 6 is H, —OH, —OR 9 , —OC(═O)R 9 , —S(═O) u R 12 , —NR 9 R 10 , C 1 -C 12 alkyl, C 1 -C 12 alkyl, C 2 -C 12 alkenyl, C 2 -C 12 alkynyl, aryl, heteroaryl, carbocyclyl, or heterocyclyl;

R 7 and R 8 are each independently selected from C 1 -C 12 alkyl, C 2 -C 12 alkenyl, C 2 -C 12 alkynyl, —C(═O)R 9 , —C(═O)NR 9 R 10 , —S(═O) u R 12 , —OH, —OR 9 , —OC(═O)R 9 , —NR 9 R 10 , aryl, heteroaryl, carbocyclyl, and heterocyclyl, or two R 7 or two R 8 groups taken together with the carbon atoms to which they are attached form an aryl, heteroaryl, carbocyclic, or heterocyclic ring;

R 9 and R 10 are each independently selected from H, C 1 -C 12 alkyl, C 2 -C 12 alkenyl, C 2 -C 12 alkynyl, aryl, heteroaryl, carbocyclyl, and heterocyclyl, or R 9 and R 10 taken together with the atoms to which they are attached form a heteroaryl or heterocyclic ring;

each instance of R 12 is independently C 1 -C 12 alkyl, C 2 -C 12 alkenyl, C 2 -C 12 alkynyl, aryl, heteroaryl, carbocyclyl, heterocyclyl, —(CH 2 ) n SiMe 3 , or —(CH 2 ) n R 9 ;

m and t are each independently an integer from 0 to 3, inclusive;

n, r, and s are each independently an integer from 0 to 4, inclusive;

u′ is 0, 1, or 2;

each instance of u is independently 0, 1, or 2; and

each of the C 1 -C 12 alkyl, C 2 -C 12 alkenyl, C 2 -C 12 alkynyl, aryl, heteroaryl, carbocyclyl, carbocyclic ring, heterocyclyl, and heterocyclic ring is independently optionally substituted.

2. The compound of claim 1 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein R 6 is H, C 1 -C 12 alkyl, C 1 -C 12 alkenyl, aryl, heteroaryl, carbocyclyl, or heterocyclyl.

3. The compound of claim 1 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein R 4 is H, —C(═O)R 9 , C 1 -C 12 alkyl, aryl, or heteroaryl.

4. The compound of claim 1 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein R 3 is C 1 -C 12 alkyl or —S(═O) u R 12 .

5. The compound of claim 1 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein R 2 and R 5 are each independently F, Br, Cl, I, C 1 -C 12 alkyl, aryl, or heteroaryl.

6. The compound of claim 1 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein R 6 is

or

and wherein X is O, NR 9 , or —S(═O) u R 12 , and v is an integer from 0 to 4, inclusive.

7. The compound of claim 6 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein R 1 is —C(═O)R 9 .

8. The compound of claim 7 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein R 9 is Me, Et, n-Pr,

or

9. The compound of claim 8 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein R 6 is

10. A pharmaceutical composition comprising a compound of claim 1 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, and a pharmaceutically acceptable excipient.

11. A method of making a compound of Formula (I):

or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein:

R 1 , R 3 , and R 4 are each independently selected from H, C 1 -C 12 alkyl, C 2 -C 12 alkenyl, C 2 -C 12 alkynyl, —C(═O)R 9 , —C(═O)NR 9 R 10 , —S(═O) u R 12 , aryl, heteroaryl, carbocyclyl, and heterocyclyl, or R 3 and R 4 taken together with the atoms to which they are attached form a heteroaryl or heterocyclic ring;

R 2 and R 5 are each independently selected from F, Cl, Br, I, —OH, —OR 9 , —OC(═O)R 9 , —S(═O) u R 12 , —NR 9 R 10 , C 1 -C 12 alkyl, C 2 -C 12 alkenyl, C 2 -C 12 alkynyl, aryl, heteroaryl, carbocyclyl, and heterocyclyl;

R 6 is H, —OH, —OR 9 , —OC(═O)R 9 , —S(═O) u R 12 , —NR 9 R 10 , C 1 -C 12 alkyl, C 1 -C 12 alkyl, C 2 -C 12 alkenyl, C 2 -C 12 alkynyl, aryl, heteroaryl, carbocyclyl, or heterocyclyl;

R 7 and R 8 are each independently selected from C 1 -C 12 alkyl, C 2 -C 12 alkenyl, C 2 -C 12 alkynyl, —C(═O)R 9 , —C(═O)NR 9 R 10 , —S(═O) u R 12 , —OH, —OR 9 , —OC(═O)R 9 , —NR 9 R 10 , aryl, heteroaryl, carbocyclyl, and heterocyclyl, or two R 7 or two R 8 groups taken together with the carbon atoms to which they are attached form an aryl, heteroaryl, carbocyclic, or heterocyclic ring:

R 9 and R 10 are each independently selected from H, C 1 -C 12 alkyl, C 2 -C 12 alkenyl, C 2 -C 12 alkynyl, aryl, heteroaryl, carbocyclyl, and heterocyclyl, or R 9 and R 10 taken together with the atoms to which they are attached form a heteroaryl or heterocyclic ring;

each instance of R 12 is independently C 1 -C 12 alkyl, C 2 -C 12 alkenyl, C 2 -C 12 alkynyl, aryl, heteroaryl, carbocyclyl, heterocyclyl, —(CH 2 ) n SiMe 3 , or —(CH 2 ) n R 9 ;

m and t are each independently an integer from 0 to 3, inclusive;

n, r, and s are each independently an integer from 0 to 4, inclusive;

u′ is 0, 1, or 2;

each instance of u is independently 0, 1, or 2; and

each of the C 1 -C 12 alkyl, C 2 -C 12 alkenyl, C 2 -C 12 alkynyl, aryl, heteroaryl, carbocyclyl, carbocyclic ring, heterocyclyl, and heterocyclic ring is independently optionally substituted:

with the proviso that the compound is not of the formula:

comprising forming a bond between the nitrogen atom at position N1 and the carbon atom at position C 8 a′, and a bond between the nitrogen atom at position N8′ and the carbon atom at position C 8 a in a compound of Formula (V):

or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein R 13 is

and R 4 is —CN, —OH, —OR 9 , —NR 9 R 10 , —S(O) u R 12 , or —P(O)(OR 9 ) 2 .

12. The method of claim 11 further comprising a radical recombination reaction of a compound of Formula (VI):

or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, to form the compound of Formula (V), or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof.

13. The method of claim 12 further comprising the extrusion of sulfur dioxide from a compound of Formula (VII):

or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, to form the compound of Formula (VI), or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof.

14. The method of claim 13 further comprising reacting a compound of Formula (III), or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof; and a compound of Formula (VIII), or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof:

to form the compound of Formula (VII), or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof.

15. The compound of claim 1 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein R 6 is heterocyclyl.

16. The compound of claim 6 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein R 6 is

X is O, and v is 0.

17. The compound of claim 1 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein R 6 is C 2 -C 12 alkenyl.

18. The compound of claim 1 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein R 6 is

19. The compound of claim 1 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein R 3 is —S(═O) u R 12 .

20. The compound of claim 19 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein u is 2.

21. The compound of claim 20 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein R 12 is —(CH 2 ) n SiMe 3 .

22. The compound of claim 1 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein R 4 is C 1 -C 12 alkyl.

23. The compound of claim 1 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein t is 1.

24. The compound of claim 1 , or a pharmaceutically acceptable salt, tautomer, or stereoisomer thereof, wherein u′ is 1.

Assignments (2)
CONFIRMATORY LICENSE Recorded Mar 27, 2019
From: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 050139/0339 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 8, 2017
From: MOVASSAGHI, MOHAMMAD; LATHROP, STEPHEN PAUL; POMPEO, MATTHEW M.; CHANG, WEN-TAU TIMOTHY
To: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
Reel/Frame 043537/0932 →
Continuity (2)
Provisional Application 62334826 · May 11, 2016
Related Publication 20170333405A1 · Nov 23, 2017