Leukocyte microRNAS for use in diagnosis and treatment of endometriosis
Methods for diagnosis and treatment of endometriosis are described. Methods utilize the recognition that leukocyte miRNAs can be dramatically dysregulated subjects suffering from endometriosis. Accordingly, leukocyte miRNAs, as well as polynucleotides encoding the miRNAs, can be utilized in the diagnosis and treatment of endometriosis.
1. A method for diagnosing and/or treating endometriosis in a subject comprising:
obtaining a peripheral blood sample from a subject;
isolating peripheral blood mononuclear cells from the peripheral blood sample;
purifying a total RNA sample from the peripheral blood mononuclear cells;
analyzing the total RNA sample from the subject to determine a quantity of each leukocyte microRNA of a set of leukocyte microRNAs present in the total RNA sample, wherein the set of leukocyte microRNAs comprises microRNA-1246, microRNA-1225-5p, microRNA-223, microRNA-451, microRNA-572, microRNA-143, microRNA-505, microRNA-155, microRNA-1281, microRNA-93, microRNA-181a-2, microRNA-181a, microRNA-342-5p, microRNA-21, microRNA-339-3p, microRNA-150, microRNA-17, microRNA-181b, microRNA-501-3p, microRNA-27a, microRNA-23a, microRNA-99b, microRNA-342-3p, let-7e, microRNA-769-5p, microRNA-320c, microRNA-1280, microRNA-766, and microRNA-339-5p;
comparing the quantity of each of the leukocyte microRNAs of the set to control quantities of each of the leukocyte microRNAs;
determining based upon the comparison that each of microRNA-1246, microRNA-1225-5p, microRNA-223, microRNA-451, microRNA-572, and microRNA-143, is overexpressed in the total RNA sample by a factor of about 3 or greater;
determining based upon the comparison that each of microRNA-505, microRNA-155, microRNA-1281, microRNA-93, microRNA-181a-2, microRNA-181a, microRNA-342-5p, microRNA-21, microRNA-339-3p, microRNA-150, microRNA-17, microRNA-181b, microRNA-501-3p, microRNA-27a, microRNA-23a, microRNA-99b, microRNA-342-3p, let-7e, microRNA-769-5p, microRNA-320c, microRNA-1280, microRNA-766, and microRNA-339-5p, is underexpressed in the total RNA sample by a factor of about 3 or greater; and
diagnosing the subject with endometriosis and/or administering a treatment for the endometriosis to the subject.
2. The method of claim 1 , wherein the total RNA sample is analyzed to obtain a quantity of genetic microRNA, primary microRNA, precursor microRNA and/or mature microRNA of each leukocyte microRNA of the set.
3. The method of claim 1 , wherein the step of analyzing the total RNA sample comprises a hybridization-based method, a quantitative real-time PCR method, and/or concentrating or isolating the microRNA from the total RNA sample.
4. The method of claim 1 , wherein the treatment comprises administration of an analgesic, administration of one or more hormones, or a surgical treatment.