HYDROGEL COMPOSITIONS FOR USE IN CELL EXPANSION AND DIFFERENTIATION
Hydrogel compositions and methods of using hydrogel compositions are disclosed. Advantageously, the hydrogel compositions offer the ability to promote cellular expansion and/or cellular differentiation of various cells.
1 . A hydrogel composition comprising an 8-arm, 20 kDa polyethylene glycol functionalized with norbornene, a crosslinking peptide, and a cell adhesion peptide.
2 . The hydrogel composition of claim 1 comprising at least 1 mM cell adhesion peptide selected from the group consisting of CRGDS (SEQ ID NO: 2), Acetylated-GCYGRGDSPG (SEQ ID NO:31), cyclic RGD (SEQ ID NO:35), CRGD-(G)13-PHSRN (SEQ ID NO:29), CPHSRN-(SG)5-RGD (SEQ ID NO:30) and IKVAV (SEQ ID NO:38).
3 . The hydrogel composition of claim 2 comprising a shear modulus range of from about 2 kPa to about 12 kPa.
4 . The hydrogel composition of claim 2 comprising a shear modulus of from about 1.8 kPa to about 33 kPa.
5 . The hydrogel composition of claim 1 comprising a concentration of polyethylene glycol of from about 36 mg/mL to about 160 mg/mL.
6 . The hydrogel composition of claim 1 comprising a concentration of polyethylene glycol of from about 36 mg/mL to about 70 mg/mL.
7 . The hydrogel composition of claim 1 comprising at least 0.25 mM of cell adhesion peptide selected from the group consisting of CRGDS (SEQ ID NO: 2), Acetylated-GCYGRGDSPG (SEQ ID NO:31), cyclic RGD (SEQ ID NO:35), CRGD-(G)13-PHSRN (SEQ ID NO:29), CPHSRN-(SG)5-RGD (SEQ ID NO:30) and IKVAV (SEQ ID NO:38).
8 . The hydrogel composition of claim 7 comprising a shear modulus of from about 3 kPa to about 16 kPa.
9 . The hydrogel composition of claim 1 further comprising an immobilized low molecular weight heparin.
10 . The hydrogel composition of claim 1 , wherein the crosslinking peptide is selected from the group consisting of KCGGPQGIWGQGCK (SEQ ID NO:27), KCGGPQGIAGQGCK (SEQ ID NO:28) and combinations thereof.
11 . The hydrogel composition of claim 1 comprising an extent of crosslinking, wherein the extent of crosslinking is from about 35% to about 75%.
12 . A hydrogel composition comprising a polyethylene glycol functionalized with norbornene, a crosslinking peptide, and at least 1 mM cell adhesion peptide selected from the group consisting of CRGDS (SEQ ID NO: 2), Acetylated-GCYGRGDSPG (SEQ ID NO:31), cyclic RGD (SEQ ID NO:35), CRGD-(G)13-PHSRN (SEQ ID NO:29), CPHSRN-(SG)5-RGD (SEQ ID NO:30) and IKVAV (SEQ ID NO:38), wherein the hydrogel composition comprises a shear modulus in the range of about 1.8 kPa to about 33 kPa.
13 . The hydrogel composition of claim 12 , wherein the shear modulus is in the range of about 2 kPa to about 12 kPa.
14 . The hydrogel composition of claim 12 comprising at least 0.25 mM of cell adhesion peptide selected from the group consisting of CRGDS (SEQ ID NO: 2), Acetylated-GCYGRGDSPG (SEQ ID NO:31), cyclic RGD (SEQ ID NO:35), CRGD-(G)13-PHSRN (SEQ ID NO:29), CPHSRN-(SG)5-RGD (SEQ ID NO:30) and IKVAV (SEQ ID NO:38).
15 . The hydrogel composition of claim 14 , wherein the shear modulus is in the range of from about 3 kPa to about 16 kPa.
16 . The hydrogel composition of claim 12 , wherein the crosslinking peptide is selected from the group consisting of KCGGPQGIWGQGCK (SEQ ID NO:27), KCGGPQGIAGQGCK (SEQ ID NO:28) and combinations thereof.
17 . A hydrogel composition comprising a polyethylene glycol functionalized with norbornene, a crosslinking peptide, and at least 1 mM cell adhesion peptide selected from the group consisting of CRGDS (SEQ ID NO: 2), Acetylated-GCYGRGDSPG (SEQ ID NO:31), cyclic RGD (SEQ ID NO:35), CRGD-(G)13-PHSRN (SEQ ID NO:29), CPHSRN-(SG)5-RGD (SEQ ID NO:30) and IKVAV (SEQ ID NO:38), wherein the hydrogel composition comprises a concentration of polyethylene glycol of from about 36 mg/mL to about 160 mg/mL.
18 . The hydrogel composition of claim 17 , wherein the concentration of polyethylene glycol is in the range of from about 36 mg/mL to about 70 mg/mL.
19 . The hydrogel composition of claim 17 comprising at least 0.25 mM of cell adhesion peptide selected from the group consisting of CRGDS (SEQ ID NO: 2), Acetylated-GCYGRGDSPG (SEQ ID NO:31), cyclic RGD (SEQ ID NO:35), CRGD-(G)13-PHSRN (SEQ ID NO:29), CPHSRN-(SG)5-RGD (SEQ ID NO:30) and IKVAV (SEQ ID NO:38).
20 . The hydrogel composition of claim 17 , wherein the crosslinking peptide is selected from the group consisting of KCGGPQGIWGQGCK (SEQ ID NO:27), KCGGPQGIAGQGCK (SEQ ID NO:28) and combinations thereof.