CELL SCAFFOLD CONSTRUCTS
The present invention relates to the regeneration, reconstruction, repair, augmentation or replacement of organs or tissue structures using scaffolds and autologous cells that are not derived from such organs or tissues.
1 . A urinary diversion for a defective bladder in a subject comprising
a) a first implantable, biocompatible construct comprising a tubular scaffold having a first end configured to connect to an abdominal wall section, a second closed end, and at least a first side opening configured to connect to a first ureter; and
b) an autologous cell population that is not derived from the defective bladder, deposited on or in a surface of the scaffold.
2 . The urinary diversion of claim 1 wherein the scaffold further comprises a second side opening configured to connect to a second ureter.
3 . The urinary diversion of claim 1 wherein the first end is configured to be positioned flush with the abdominal wall.
4 . The urinary diversion of claim 3 wherein the first end is configured to be sutured to the skin of the subject.
5 . The urinary diversion of claim 4 wherein the first end is configured to form a stoma.
6 . The urinary diversion of claim 5 wherein the stoma further comprises a stoma button.
7 . The urinary diversion of claim 5 , wherein the scaffold further comprises a washer ring configured to form a stoma.
8 . The urinary diversion of claim 1 , wherein the biocompatible scaffold is biodegradable.
9 . The urinary diversion of claim 1 , wherein the scaffold comprises a material selected from the group consisting of polyglycolic acid, polylactic acid, and a copolymer of polyglycolic acid and polylactic acid.
10 . The urinary diversion of claim 1 , wherein the cell population is a smooth muscle cell population.
11 . The urinary diversion of claim 1 wherein the diversion is a replacement for the defective bladder.
12 . The urinary diversion of claim 1 , wherein the diversion is temporary.
13 . The urinary diversion of claim 1 , wherein the diversion is permanent.
14 . The urinary diversion of claim 1 , wherein the tubular scaffold has a rectangular cross-section configuration.
15 . The urinary diversion of claim 1 , wherein the tubular scaffold has a triangular cross-section configuration.
16 . The urinary diversion of claim 1 , wherein the tubular scaffold has a circular cross-section configuration.
17 . The urinary diversion of claim 1 , wherein the diversion is free of urothelial cells.
18 . A method of preparing a urinary diversion construct for a defective bladder in a subject in need comprising
a) providing a first implantable biocompatible scaffold comprising a tubular scaffold having a first end configured to contact an abdominal wall section, a second closed end, and at least a first side opening configured to connect to a first ureter; and
b) depositing an autologous cell population that is not derived from the defective bladder on or in a first area of said scaffold to form a urinary diversion construct.
19 . The method of claim 18 , wherein the scaffold further comprises a second side opening configured to connect to a second ureter.
20 .- 35 . (canceled)
36 . A method of providing a urinary diversion for a defective bladder in a subject in need comprising implanting into said subject a urinary diversion construct comprising (a) a tubular scaffold having a first end configured to contact an abdominal wall section, a second closed end, and at least a first side opening configured to connect to a first ureter; and (b) an autologous cell population that is not derived from the defective bladder, deposited on or in a surface of the scaffold, for the formation of the urinary diversion.
37 .- 55 . (canceled)