Methods for increasing intracellular activity of Hsp70
The present invention relates to a bioactive agent capable of increasing the intracellular concentration and/or activity of Hsp70 for use in the treatment of a lysosomal storage disease which arise from a defect in an enzyme whose activity is not directly associated with the presence of lysosomal BMP as a co-factor; such as glycogen storage diseases, gangliosidoses, neuronal ceroid lipofuscinoses, cerebrotendinous cholesterosis, Wolman's disease, cholesteryl ester storage disease, disorders of glycosaminoglycan metabolism, mucopolysaccharidoses, disorders of glycoprotein metabolism, mucolipidoses, aspartylglucosaminuria, fucosidosis, mannosidoses, and sialidosis type II.
1. A method of treatment of a neuronal ceroid lipofuscinosis,
said method comprising administering to an individual in need thereof a bioactive agent capable of increasing the intracellular concentration of Hsp70, wherein said bioactive agent is selected from the group consisting of Hsp70; and a functional fragment or variant of Hsp70 having at least 95% sequence identity to Hsp70.
2. The method according, to claim 1 , wherein said neuronal ceroid lipofuscinosis results from a defect in an enzyme, wherein the activity of said enzyme is not directly associated with the presence of lysosomal BMP as a co-factor.
3. The method according to claim 1 , wherein said neuronal ceroid lipofuscinosis is selected from the group consisting of Batten disease (Spielmeyer-Vogt disease), Bielschowsky-Jansky disease, Kufs disease, and Santavuori-Haltia disease.
4. The method according to claim 1 , wherein said bioactive agent is administered in combination with at least one other treatment modality.
5. The method according to claim 4 , wherein said at least one other treatment modality is selected from the group consisting of enzyme replacement therapy (ERT), pain relievers, corticosteroids, substrate reduction therapy, a transplantation and physical therapy.
6. The method according, to claim 1 , wherein said Hsp70 is derived from a mammal selected from the group consisting of human ( Homo sapiens ), mouse ( Mus musculus ), cow, dog, rat, ferret, pig, sheep, and monkey.
7. The method according to claim 1 , wherein said bioactive agent is Hsp70.
8. The method according to claim 1 , wherein said bioactive agent is recombinant Hsp70 (rHsp70).
9. The method according to claim 1 , wherein said bioactive agent is a functional fragment or variant of Hsp70 having at least 95% sequence identity to Hsp70.
10. The method according to claim 1 , wherein said functional fragment or variant of Hsp70 having at least 95% sequence identity to Hsp70 comprises all or part of the ATPase domain of Hsp70; and/or comprises tryptophan at amino acid position 90 of the Hsp70 ATPase domain.
11. The method according to claim 1 , wherein administering said Hsp70 or said functional fragment or variant of Hsp70 having at least 95% sequence identity to Hsp70 comprises administering an expression vector encoding said Hsp70 or said functional fragment or variant of Hsp70 having at least 95% sequence identity to Hsp70.