Solid phase peptide synthesis processes and associated systems
Systems and processes for performing solid phase peptide synthesis are generally described. Solid phase peptide synthesis is a known process in which amino acid residues are added to peptides that have been immobilized on a solid support. In certain embodiments, the inventive systems and methods can be used to perform solid phase peptide synthesis quickly while maintaining high yields. Certain embodiments relate to processes and systems that may be used to heat, transport, and/or mix reagents in ways that reduce the amount of time required to perform solid phase peptide synthesis.
1. A peptide synthesizer system, comprising:
a first reagent reservoir connected to a first channel;
a second reagent reservoir connected to a second channel;
a pre-heat channel downstream of and fluidically connected to the first channel and the second channel;
a reactor downstream of and connected to the pre-heat channel;
a heat exchanger in thermal communication with the pre-heat channel and the reactor;
a waste channel downstream of and connected to the reactor; and
a UV detector in optical communication with the waste channel.
2. The peptide synthesizer system of claim 1 , further comprising a second pump downstream of and in fluid communication with the second channel, wherein the second pump is upstream of and in fluid communication with the pre-heat channel.
3. The peptide synthesizer system of claim 1 , wherein the heat exchanger comprises a liquid bath, a resistive heater, or a gas convection-based heating element.
4. The peptide synthesizer system of claim 1 , comprising a solid support positioned within the reactor.
5. The peptide synthesizer system of claim 1 , further comprising a first pump downstream of and in fluid communication with the first channel, wherein the first pump is upstream of and in fluid communication with the pre-heat channel.
6. The peptide synthesizer system of claim 5 , further comprising a third reagent reservoir connected to a third channel upstream of and fluidically connected to the pre-heat channel.
7. The peptide synthesizer system of claim 6 , further comprising a first reagent selection valve upstream of and connected to the pump, wherein the first reagent selection valve is downstream of and fluidically connected to the first channel and the third channel.