Method and apparatus for detection of foam in specimen containers
The present invention is directed to a method and apparatus for detecting foam in a specimen container. The method includes the following steps: transporting a specimen container into a locator well; centering the specimen container in the locator well; rotating the specimen container around a vertical axis in the locator well; imaging the specimen container during the rotation; analyzing an image of the specimen container captured during the rotation; and detecting foam in the specimen container based on the analysis of the image. An apparatus configured to perform the steps is also provided. The method and apparatus may be used in conjunction with a system for automatically determining whether a sample is positive for microorganism growth.
1. An apparatus for detecting foam in a specimen container; the apparatus comprising:
an automated loading mechanism for transporting a specimen container containing a fluid into a locator well;
a container locator device comprising a rotatable disk containing one or more locator wells, each locator well capable of holding a single specimen container, wherein said rotatable disk is rotatable in a horizontal plane about a vertical axis to move said specimen container to one or more container work-flow stations;
a rotatable turntable at an imaging station of one of the container work-flow stations, said rotatable turntable configured to rotate said specimen container in a horizontal plane about a vertical axis in said locator well;
at least one centering device configured to center the specimen container in the locator well at the imaging station;
an imaging device positioned proximate to the imaging station; and
a server comprising a processor and memory, the memory having computer readable program code, and the processor programmed to:
image the specimen container during the rotation;
analyze an image of the specimen container captured during the rotation; and
detect foam in the specimen container based on the analysis of the image.
2. The apparatus of claim 1 , wherein the centering device is selected from the group consisting of a cup positioned in a center of the locator well at the imaging station and a fence configured to direct the specimen container to the center of the locator well at the imaging station.
3. The apparatus of claim 1 , further comprising a motor configured to rotate the specimen container at a nominal rotation period of about 1.8 sec / rotation.
4. The apparatus of claim 1 , further comprising a light bar positioned to illuminate the specimen bottle during imaging of the specimen container.
5. The apparatus of claim 4 , wherein the processor is further programmed to perform dynamic exposure of the imaging device to the light bar to compensate for different illumination board output levels during imaging.
6. The apparatus of claim 4 , wherein the light bar produces blue light to enhance contrast between the fluid in the specimen bottle and a background of the locator well.
7. The apparatus of claim 4 , wherein the light bar is positioned at an angle relative to the vertical axis to optimize the illumination for the detection of foam.
8. The apparatus of claim 1 , wherein the well comprises a surface configured to enhance contrast with the fluid in the specimen container.
9. The apparatus of claim 1 , wherein the processor is further programmed to:
stitch a plurality of images captured during the rotation into a single image, and
display the single image of the specimen container to a user.
10. The apparatus of claim 1 , wherein the processor is further programmed to:
determine a first distance between a bottom of the specimen container and a marker on a side of the specimen container;
determine a second distance between the bottom of the specimen container and a liquid level in the specimen container; and
determine a difference between the first distance and the second distance.