Liquid chromatograph and method for correcting detector output value fluctuation of liquid chromatograph
A minimum peak is determined from analysis results, a correction wavelength at which an S/N ratio of the minimum peak is greatest is determined, and the determined correction wavelength is used to execute correction of the minimum peak. A plurality of detector output value correction method are registered in a processor, correction method is selected from default correction method or from among a plurality of preset correction methods according to an object to perform correction.
1. A method for correcting detector output value of a liquid chromatograph, the method comprising:
selecting an analysis condition for using a liquid chromatograph, the analysis condition being selected from preset recipes that are registered in advance according to an examination object, the analysis condition being a condition to be set with respect to devices included in the liquid chromatograph such as a pump, an auto sampler, a column oven, and a detector,
starting analysis according to the analysis condition that is selected in the previous step, measuring a characteristic of a sample using the detector, the characteristic including at least one of an absorbency and a fluorescence amount, and storing and displaying a signal strength of the detector indicating the characteristic of the sample in relation to time as analysis results;
obtaining a chromatogram;
recognizing peaks included in the chromatogram;
determining a most minute peak of the signal strength of the analysis results in peaks which are recognized as a correction target peak;
determining correction candidate wavelengths with respect to the correction target peak;
performing a correction process on detector output value fluctuation based on correction method which is selected from among a plurality of preset correction methods; and
outputting the analysis conditions, the correction condition, and corrected results of the characteristic of the sample.
2. The method of claim 1 further comprising:
selecting one wavelength of a correction wavelength at which an S/N ratio, which is a ratio between a detector output signal of the correction target peak and a detector output signal in the correction wavelength, is greatest and correcting the detector output signal of the correction target peak.
3. The method of claim 1 further comprising:
selecting one of a correction wavelength at which reproducibility of an S/N ratio is most favorable and correcting a detector output signal of the correction target peak.
4. The method of claim 1 further comprising:
selecting a plurality of correction wavelengths at which reproducibility of an S/N ratio is most favorable and correcting a detector output signal of the correction target peak using an average value of the detector output values in the plurality of selected wavelengths.
5. The method of claim 1 further comprising:
selecting each correction wavelength such that an S/N ratio of the correction target peak of each measurement target component is greatest and correcting a detector output signal of each of the correction target peaks.
6. The method of claim 1 further comprising:
selecting a plurality of correction wavelengths at which reproducibility of an S/N ratio of the correction target peak of each measurement target component is most favorable and correcting a detector output signal of the correction target peak using an average value of the detector output values in the plurality of selected wavelengths.
7. The method of claim 1 further comprising:
registering absorption wavelengths of a mobile phase which is used in liquid chromatography in advance as a database;
selecting correction wavelength from the database in accordance with the mobile phase being selected; and
correcting a detector output signal of the correction target peak using the selected correction wavelength.