Escorted and functionalized guides for CRISPR-Cas systems
View Patent ↗The present invention generally relates to CRISPR systems or complexes, such as those with an escorted guide RNA.
1. A non-naturally occurring or engineered composition comprising an escorted single CRISPR-Cas9 guide, comprising:
a guide sequence capable of hybridizing to a target sequence in a genomic locus of interest in a cell; and,
an escort aptamer sequence fused directly to one or more of a tetraloop or a stem loop of the guide sequence,
wherein the escort aptamer sequence comprises binding affinity for an aptamer ligand on or in the cell, or the escort aptamer sequence is responsive to a localized aptamer effector on or in the cell,
wherein the aptamer ligand or effector is present at a location or time of interest on or in the cell, and
wherein binding of the escort aptamer sequence to the aptamer ligand directs the escorted single guide to the location of interest in the cell.
2. The composition of claim 1 , wherein the escort aptamer sequence changes conformation in response to an interaction with the aptamer ligand or effector in the cell; or wherein the escort aptamer sequence is complementary to a target miRNA, and binding of the escort aptamer sequence to the target miRNA results in cleavage of the escorted single guide by an RNA-induced silencing complex (RISC) within the cell.
3. The composition of claim 1 , wherein the aptamer ligand is localized in a location or compartment of the cell; or wherein the localized aptamer ligand is on or in a membrane of the cell.
4. The composition of claim 1 , wherein the aptamer ligand or effector on or in the cell is spatially restricted to a location which comprises a cell nucleus or mitochondrion, or wherein the location of interest in the cell is the interior of the cell and the aptamer ligand comprises a cell surface aptamer ligand.
5. The composition of claim 4 , wherein the target sequence is a Cas9 gene or RNA sequence.
6. The composition of claim 1 , wherein the escorted single guide further comprises an aptamer linking sequence, operably linking the escort sequence to the guide sequence; or wherein the escorted single guide comprises one or more photolabile bond(s).
7. The composition of claim 1 , wherein the escort aptamer sequence is from 10 to 200 nucleotides in length; or wherein the guide sequence is 10-30 nucleotides long; or wherein the guide sequence comprises a seed sequence of 2 to 18 nucleotides, optionally positioned within 2, 3 or 4 nucleotides from the 3′ end of the guide sequence.
8. The composition of claim 7 , wherein the seed sequence is at least 75%, at least 90%, or 100% complementary to the target sequence.
9. The composition of claim 1 , wherein the escorted single guide comprises more than one escort aptamer sequence; or wherein the guide sequence is operably linked to a tracr mate sequence.
10. The composition of claim 9 , wherein the tracr mate sequence is operably linked to the 3′ end of the guide sequence.
11. The composition of claim 1 , wherein gene editing activity of the escorted single CRISPR-Cas9 guide is at least 25% higher compared to a counterpart single CRISPR-Cas9 guide that does not have the escorted aptamer sequence.
12. A non-naturally occurring or engineered CRISPR-Cas9 complex composition comprising the escorted single guide of claim 1 , and a CRISPR-Cas9 enzyme.
13. A non-naturally occurring or engineered composition comprising:
the escorted single guide of claim 1 , and
a CRISPR Cas9 protein comprising at least one or more nuclear localization sequences,
wherein the CRISPR protein comprises at least one mutation, such that the CRISPR-Cas9 protein comprises no more than 5% of the nuclease activity of the CRISPR-Cas9 protein not having the at least one mutation, or wherein the CRISPR-Cas9 protein comprises a nuclease activity diminished by at least 97%, or 100% as compared with the CRISPR Cas9-protein not having the at least one mutation.
14. The composition of claim 13 , wherein the CRISPR Cas9 protein comprises two or more mutations of D10, E762, H840, N854, N863, or D986 according to SpCas9 protein, or comprises at least one mutation wherein at least H840 is mutated.
15. The composition of claim 14 , wherein the CRISPR Cas9 protein comprises two or more mutations comprising D10A, E762A, H840A, N854A, N863A or D986A according to SpCas9 protein, or at least one mutation comprising H840A; or wherein the CRISPR-Cas9 protein comprises H840A, or D10A and H840A, or D10A and N863A, according to SpCas9 protein.
16. The composition of claim 13 , wherein the composition is in a cell, in a eukaryotic cell, in a mammalian cell, or in a human cell; or wherein the composition comprises a CRISPR-Cas9 complex having at least three functional domains, at least one of which is associated with the CRISPR protein and at least two of which are associated with escorted single guide.
17. A method for introducing genomic locus event comprising administration to a host or expression in a host in vivo of the composition of claim 1 , whereby the genomic locus event occurs at the location of interest.
18. The method of claim 17 , wherein the genomic locus event comprises gene activation, gene inhibition, or cleavage in the locus; or wherein the genomic locus event modifies gene expression in a cell.
19. The method of claim 17 , wherein the host is a eukaryotic cell, or a mammalian cell, or a non-human eukaryote, or a non-human mammal, or a mouse.
20. The method of claim 17 , which comprises delivery of the composition of claim 1 or nucleic acid molecule(s) coding therefor, wherein said nucleic acid molecule(s) are operatively inked to regulatory sequence(s) for expression in vivo.
21. The method according to claim 20 wherein the delivery is via a lentivirus, an adenovirus, or an adeno-associated virus (AAV) vector.