Diagnostic assays and kits for detection of folate receptor 1
The invention generally relates to antibodies that bind to human folate receptor 1 and diagnostic assays for folate receptor 1-based therapies. Methods of using the antibodies to monitor therapy are further provided.
1. A nucleic acid molecule comprising a polynucleotide sequence encoding an antibody or antigen-binding fragment thereof that specifically binds to FOLR1 and comprises variable heavy chain CDR1, CDR2, and CDR3 comprising the amino acid sequences of SEQ ID NOS:1, 2, and 3, respectively, and variable light chain CDR1, CDR2, and CDR3 comprising the amino acid sequences of SEQ ID NOS:13, 14, an 15, respectively.
2. A vector comprising the nucleic acid molecule of claim 1 .
3. The vector of claim 2 , wherein said vector further comprises a promoter binding site and optionally an enhancer sequence.
4. The vector of claim 2 , wherein said vector is a plasmid, a virus, a phage, a bacterium or a viroid.
5. A cell producing an antibody or antigen-binding fragment thereof encoded by the nucleic acid molecule of claim 1 .
6. The cell of claim 5 , wherein said cell is a bacterial cell or a eukaryotic cell.
7. A method of making an antibody or antigen-binding fragment thereof, comprising (a) culturing the cell of claim 5 ; and (b) isolating the antibody or antigen-binding fragment thereof from said cultured cell.
8. The nucleic acid molecule of claim 1 , wherein the antibody or antigen-binding fragment thereof comprises the variable heavy chain (VH) polypeptide of SEQ ID NO:25 and the variable light chain (VL) polypeptide of SEQ ID NO:29.
9. The nucleic acid molecule of claim 1 , wherein the antibody or antigen-binding fragment thereof comprises the full-length heavy chain polypeptide of SEQ ID NO:33 and the full-length light chain polypeptide of SEQ ID NO:37.
10. The nucleic acid molecule of claim 1 , wherein the antibody or antigen-binding fragment thereof is non-human, humanized, chimeric, or resurfaced.
11. The nucleic acid molecule of claim 1 , wherein the antibody or antigen-binding fragment thereof is a full length antibody.
12. The nucleic acid molecule of claim 1 , wherein the antibody or antigen-binding fragment thereof is an antigen-binding fragment.
13. The nucleic acid molecule of claim 1 , wherein the antibody or antigen-binding fragment thereof comprises a Fab, Fab′, F(ab′)2, Fd, single chain Fv or scFv, disulfide linked Fv, V-NAR domain, IgNar, intrabody, IgGΔCH2, minibody, F(ab′)3, tetrabody, triabody, diabody, single-domain antibody, DVD-Ig, Fcab, mAb2, (scFv)2, or scFv-Fc.
14. The nucleic acid molecule of claim 1 , wherein the antibody or antigen-binding fragment thereof binds to a human FOLR1 with a Kd of about 1.0 nM to about 10 nM.
15. A nucleic acid molecule comprising a polynucleotide sequence encoding a polypeptide comprising SEQ ID NO:25.
16. A nucleic acid molecule comprising a polynucleotide sequence encoding a polypeptide comprising SEQ ID NO:29.
17. A cell comprising the nucleic acid molecule of claim 15 ;
further comprising a second nucleic acid molecule comprising a polynucleotide sequence encoding a polypeptide comprising SEQ ID NO:29.