Factor V/Va-targeting aptamer compositions and methods of using the same
Provided herein are Factor V/Factor Va-targeting aptamer compositions and antidote compositions targeting such aptamer compositions. Methods for preventing blood clots using such compositions are also provided.
1. An aptamer comprising
SEQ ID NO: 1,
SEQ ID NO: 2,
a polynucleotide having at least 85% sequence identity to SEQ ID NO: 1,
a polynucleotide having at least 85% sequence identity to SEQ ID NO: 2,
a polynucleotide comprising from 5′ to 3′ the nucleotide sequence GGA, a first stem forming region consisting of 7 nucleotides, a first loop region consisting of the nucleotide sequence AA, a second stem forming region consisting of 4 nucleotides, a second loop region consisting of the nucleotide sequence A, a third stem forming region consisting of 4 nucleotides, a third loop region consisting of the nucleotide sequence AAUG, a fourth stem forming region consisting of 4 nucleotides and capable of forming a stem with the third stem forming region, a fourth loop region consisting of the nucleotide sequence CUU, a fifth stem forming region consisting of 3 nucleotides, a fifth loop region consisting of the nucleotide sequence AGAC, a sixth stem forming region consisting of 3 nucleotides and capable of forming a stem with the fifth stem forming region, a sixth loop region consisting of the nucleotide sequence UCGCU, a seventh stem forming region consisting of 4 nucleotides and capable of forming a stem with the second stem forming region, an eighth stem forming region consisting of 7 nucleotides and capable of forming a stem with the first stem forming region, or
a polynucleotide comprising from 5′ to 3′ a first stem forming region consisting of 6 nucleotides, a first loop region consisting of the nucleotide sequence AAC, a second stem forming region consisting of 4 nucleotides, a second loop region consisting of the nucleotide sequence AAUUAC, a third stem forming region consisting of 4 nucleotides and capable of forming a stem with the second stem forming region, a third loop region consisting of the nucleotide sequence CUUG, a fourth stem forming region consisting of 4 nucleotides, a fourth loop region consisting of the nucleotide sequence A, a fifth stem forming region consisting of 2 nucleotides, a fifth loop region consisting of the nucleotide sequence ACU, a sixth stem forming region consisting of 2 nucleotides and capable of forming a stem with the fifth stem forming region, a seventh stem forming region consisting of 4 nucleotides and capable of forming a stem with the fourth stem forming region, and an eighth stem forming region consisting of 6 nucleotides and capable of forming a stem with the first stem forming region.
2. The aptamer of claim 1 , wherein the aptamer comprises SEQ ID NO: 3, SEQ ID NO: 4, a polynucleotide having at least 85% sequence identity to SEQ ID NO: 3, or a polynucleotide having at least 85% sequence identity to SEQ ID NO: 4.
3. The aptamer of claim 1 , wherein the aptamer binds to Factor V or Factor Va.
4. The aptamer of claim 1 , wherein the polynucleotide is an RNA polynucleotide.
5. The aptamer of claim 1 , wherein the polynucleotide comprises at least one nucleotide base modification selected from the group consisting of a 2′fluoro modification, a 2′O-methyl modification, a 5′ modification, and a 3′modification.
6. The aptamer of claim 1 , wherein the polynucleotide comprises a 5′ linker and/or a 3′ linker.
7. The aptamer of claim 1 , wherein the polynucleotide further comprises a stability agent.
8. The aptamer of claim 7 , wherein the stability agent is selected from the group consisting of polyethylene glycol (PEG), cholesterol, albumin, and Elastin-like polypeptide.
9. The aptamer of claim 8 , wherein the stability agent is linked to the 5′ end of the polynucleotide.
10. The aptamer of claim 8 , wherein the polynucleotide and the stability agent are linked by a covalent bond.
11. The aptamer of claim 8 , wherein the polynucleotide and the stability agent are linked by a tag system.
12. The aptamer of claim 11 , wherein the tag system is selected from the group consisting of biotin/streptavidin, and biotin/NeutrAvidin.
13. A dimer, trimer, or tetramer comprising the aptamer of claim 1 .
14. An antidote comprising a polynucleotide having a nucleotide sequence reverse complementary to and capable of hybridizing to at least 8 nucleotides of the aptamer of claim 1 .
15. A pharmaceutical composition comprising a pharmaceutical carrier and the aptamer of claim 1 .
16. A method for preventing blood clot formation in a subject comprising administering to the subject the aptamer of claim 1 in a therapeutically effective amount to prevent blood clot formation in the subject.
17. The method of claim 16 , wherein the subject suffers from FV Leiden, atrial fibrillation or is at risk of having a Deep Vein Thrombosis, a stroke, a heart attack, or a pulmonary embolism.
18. The method of claim 16 , further comprising administering to the subject an antidote in a therapeutically effective amount to neutralize the aptamer.
19. The method of claim 18 , wherein the antidote comprises the antidote of claim 14 .
20. The method of claim 16 , wherein the subject is a human.