Resistance alleles in soybean
The present invention relates to methods and compositions for identifying, selecting and/or producing a soybean plant or germplasm having iron deficiency chlorosis tolerance. A soybean plant, part thereof and/or germplasm, including any progeny and/or seeds derived from a soybean plant or germplasm identified, selected and/or produced by any of the methods of the present invention is also provided.
1. A method of producing a soybean plant tolerant to iron deficiency chlorosis (IDC), the method comprising the steps of:
(a) isolating a nucleic acid from a soybean plant or part;
(b) detecting in said nucleic acid, the presence of a single nucleotide polymorphism (SNP) marker associated with IDC tolerance in a soybean plant, wherein said marker is located within a chromosomal interval comprising physical positions that correspond to 2850183-8685663 on soybean chromosome 2 in a reference genome of Williams 82, and further wherein said chromosomal interval comprises (i) a T allele at a physical position that corresponds to 3091839 on soybean chromosome 2 in the reference genome of Williams 82; (ii) a G allele at a physical position that corresponds to 8685663 on soybean chromosome 2 in the reference genome of Williams 82; (iii) an A allele at a physical position that corresponds to 4901534 on soybean chromosome 2 in the reference genome of Williams 82; and (iv) a G allele at a physical position that corresponds to 7472790 on soybean chromosome 2 in the reference genome of Williams 82;
(c) identifying and/or selecting an iron deficiency chlorosis (IDC) tolerant soybean;
(d) crossing the soybean plant of (c) with a second soybean plant not having said SNP marker associated with IDC tolerance in its genome;
(e) collecting seed from the cross in step (d);
(f) growing a progeny soybean plant from said seed which comprises said SNP marker associated with IDC tolerance in its genome, thereby producing a soybean plant with increased tolerance to IDC.
2. The method of claim 1 , wherein the nucleic acid of (b) comprises any one of SEQ ID NOs: 7-10, 61 or 74.
3. The method of claim 1 wherein IDC tolerance is exhibited by reduced yellow flash symptoms.
4. The method of claim 2 , wherein the iron deficiency chlorosis (IDC) tolerant soybean plant or part thereof is identified through use of any one of nucleotide probes comprising the nucleotide sequence of any one of SEQ ID NOs: 25-28, 43-46, 143, 156, 225, or 238.
5. The method of claim 2 , wherein the iron deficiency chlorosis (IDC) tolerant soybean plant or part thereof is identified through use of a PCR primer pair that anneals to any one of SEQ ID NOs: 7-10, 61 or 74, wherein the primer pair is capable of initiating DNA polymerization by a DNA polymerase on a Glycine max nucleic acid template to generate a Glycine max amplicon.
6. The method of claim 5 , wherein the amplicon comprises a nucleotide sequence that is distinguishing for the presence or absence of alleles selected from the group consisting of: (i) the T allele at the physical position that corresponds to 3091839 on soybean chromosome 2 in the reference genome of Williams 82; (ii) a G allele at the physical position that corresponds to 8685663 on soybean chromosome 2 in the reference genome of Williams 82; (iii) an A allele at the physical position that corresponds to 4901534 on soybean chromosome 2 in the reference genome of Williams 82; (iv) a G allele at the physical position that corresponds to 7472790 on soybean chromosome 2 in the reference genome of Williams 82, and any combination of (i) through (iv).