Separation of multi-component fluid through ultrasonic acoustophoresis
An acoustic standing wave is utilized to separate components from a multi-component fluid, such as oil from an oil-water mixture, in a fluid flow scheme with an acoustophoresis device. For example, the flow scheme and device allows for trapping of the oil as the oil coalesces, agglomerates, and becomes more buoyant than the water.
1. A method for continuously separating cells or particles from a host fluid, comprising:
flowing the host fluid through an acoustophoresis device that comprises:
one or more device inlets at a first end of the device;
a flow chamber downstream of the one or more device inlets, the flow chamber including:
a flow chamber inlet at a first end for receiving the fluid flow,
a flow chamber outlet at a second end opposite the first end,
at least one ultrasonic transducer including a piezoelectric material configured to be driven to generate a multi-dimensional acoustic standing wave in the flow chamber, and
a reflector located opposite the at least one ultrasonic transducer; and
one or more device outlets located at a second end of the device downstream of the flow chamber outlet;
driving the at least ultrasonic transducer to create the multi-dimensional acoustic standing wave in the flow chamber and separating the cells or particles from the host fluid.
2. The method of claim 1 , wherein the cells or particles exit the device through the one or more device outlets located at the second end of the device.
3. The method of claim 1 , wherein the host fluid exits the device through a first device outlet located at the first end of the device and separated from the one or more device inlets by a longitudinal sidewall.
4. The method of claim 3 , wherein the device has a length L from the one or more device inlets to a bottom of the longitudinal sidewall, and a ratio of the length L to the first diameter is less than 1.
5. The method of claim 1 , wherein the device operates at a flow rate of up to 3 gallons/minute.
6. The method of claim 1 , wherein the first end of the device has a circular cross-section and the flow chamber has a rectangular cross-section.
7. The method of claim 1 , wherein the multi-dimensional acoustic standing wave results in an acoustic radiation force with an axial force component and a lateral force component that are of the same order of magnitude.
8. The method of claim 1 , wherein the transducer comprises:
a housing including a top end, a bottom end, and an interior volume; and
a crystal at the bottom end of the housing including an exposed exterior surface and an interior surface, the crystal being able to vibrate when driven by a voltage signal.
9. The method of claim 8 , wherein no backing layer is present within the housing of the transducer, and an air gap is present in the interior volume between the crystal and a top plate at the top end of the housing.
10. The method of claim 8 , wherein the transducer further comprises a backing layer contacting the interior surface of the crystal, the backing layer being made of a substantially acoustically transparent material.
11. The method of claim 1 , wherein the flow chamber further comprises a transparent window for viewing the interior of the flow chamber.
12. An acoustophoresis device, comprising:
one or more device inlets at a first end of the device, the first end including a first diameter for receiving fluid flow;
a chamber downstream of the one or more device inlets, the chamber including:
at least one ultrasonic transducer, the ultrasonic transducer including a piezoelectric material configured to be driven to generate a multi-dimensional acoustic standing wave in the chamber, and
a reflector located opposite side from the at least one ultrasonic transducer;
one or more device outlets located at a second end of the device downstream of the chamber.
13. The device of claim 12 , wherein the device includes a plurality of device inlets spaced about the first end of the device.
14. The device of claim 12 , wherein the piezoelectric material of the at least one ultrasonic transducer has a rectangular shape.
15. The device of claim 12 , wherein the reflector has a non-planar surface.
16. The device of claim 12 , wherein the first end of the device has a circular cross-section and the flow chamber has a rectangular cross-section.
17. The device of claim 12 , wherein the transducer comprises:
a housing including a top end, a bottom end, and an interior volume; and
a crystal at the bottom end of the housing including an exposed exterior surface and an interior surface, the crystal being able to vibrate when driven by a voltage signal.
18. The device of claim 12 , wherein the flow chamber further comprises a transparent window for viewing the interior of the flow chamber.
19. The device of claim 1 , wherein the device has a length L from the one or more device inlets to a bottom of the longitudinal sidewall, and a ratio of the length L to the first diameter is less than 12.
20. The device of claim 12 , wherein the flow chamber has a plurality of the ultrasonic transducers located on the wall of the flow chamber.