Process for enhancing the viable counts of lactic acid bacteria and useful compositions thereof
Disclosed herein is (i) a method related to the growth promotional activity of plant based fibers on Bacillus coagulans MTCC 5856; (ii) the combination of plant based fibers and Bacillus coagulans MTCC 5856 to inhibit Gram Negative pathogenic bacteria; and (iii) the production of short chain fatty acids (SCFA) by Bacillus coagulans MTCC 5856 using plant based fibers.
1. A method comprising co-culturing Bacillus coagulans MTCC 5856 with plant fibers selected from the group consisting of Trigonella foenum - graecum (fenugreek) seed fibers, Lycium barbarum seed fibers, Linum usitatissimum (Flax) seed fibers, Cocos nucifera (Coconut) fibers, Zingiber officinale (Ginger) rhizome fibers, Emblica officinalis (Amla) fruit fibers, Plantago ovata ( Psyllium ) fibers, and Vaccinium oxycoccos (Cranberry) seed fibers.
2. The method according to claim 1 , wherein the viable colony count of Bacillus coagulans MTCC 5856 is increased by the co-culturing compared to Bacillus coagulans MTCC 5856 cultured in the absence of the plant fibers.
3. The method according to claim 1 , further comprising producing a composition comprising the Bacillus coagulans MTCC 5856 and the plant fibers.
4. A method of inhibiting pathogenic Gram Negative bacteria, comprising:
obtaining a composition that was produced by co-culturing Bacillus coagulans MTCC 5856 with plant fibers selected from the group consisting of Trigonella foenum - graecum (fenugreek) seed fibers, Lycium barbarum seed fibers, Linum usitatissimum (Flax) seed fibers, Cocos mucifera (Coconut) fibers, Zingiber officinale (Ginger) rhizome fibers, Emblica officinalis (Amla) fruit fibers, Plantago ovata ( Psyllium ) fibers, and Vaccinium oxycoccos (Cranberry) seed fibers; and
contacting the pathogenic Gram Negative bacteria with the obtained composition to inhibit the pathogenic Gram Negative bacteria.
5. The method according to claim 4 , wherein the pathogenic Gram Negative bacteria is E. coli.
6. The method according to claim 1 , wherein the co-culturing is performed under fermenting conditions to produce short chain fatty acids.
7. The method according to claim 6 , wherein the short chain fatty acids are one or more selected from the group consisting of acetic acid, propionic acid, and butyric acid.
8. The method according to claim 6 , wherein the Bacillus coagulans MTCC 5856 and the plant fibers are fermented under anaerobic conditions.
9. A method for treatment and/or prophylaxis of diet-induced obesity and/or insulin resistance, comprising:
obtaining a composition that was produced by co-culturing Bacillus coagulans MTCC 5856 with plant fibers selected from the group consisting of Trigonella foenum - graecum (fenugreek) seed fibers, Lycium barbarum seed fibers, Linum usitatissimum (Flax) seed fibers, Cocos nucifera (Coconut) fibers, Zingiber officinale (Ginaer) rhizome fibers, Emblica officinalis (Amla) fruit fibers, Plantago ovata ( Psyllium ) fibers, and Vaccinium oxycoccos (Cranberry) seed fibers; and
administering an effective amount of the obtained composition to a subject in need of treatment and/or prophylaxis of diet-induced obesity and/or insulin resistance.