Brassica GAT event and compositions and methods for the identification and/or detection thereof
Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.
1. A method of detecting the presence of DNA corresponding to a DP-073496-4 event in a sample, the method comprising:
(a) contacting the sample with a polynucleotide probe that hybridizes under stringent hybridization conditions with DNA from brassica event DP-073496-4, wherein the probe binds to a junction sequence of the event DP-073496-4, the junction sequence selected from the group consisting of SEQ ID NOS: 12 and 13;
(b) subjecting the sample and probe to stringent hybridization conditions; and
(c) detecting hybridization of the probe to the DNA, wherein detection of hybridization indicates the presence of the DP-073496-4 event.
2. The method of claim 1 , wherein said sample comprises brassica tissue.
3. The method of claim 1 , wherein the detection of hybridization indicates seed purity or seed lot purity.