IP Library › Granted Patent US 10,428,122
Granted Patent B2
US 10,428,122 · App. 15/624,853 · Granted Oct 1, 2019

Tuberculosis compositions and methods of treating or preventing tuberculosis

Inventors: Ravi P. Anantha (Rockville, MD); Thomas G. Evans (Rockville, MD); Aurelio M. Bonavia (Rockville, MD)
Assignee: International AIDS Vaccine Initiative, Inc.
C07K14/35A61K39/04A61P31/06A61K2039/53A61K2039/55561C07K2319/00
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Quick Facts
Patent No.
US 10,428,122
App. No.
15/624,853
Granted
Oct 1, 2019
Kind
B2
Abstract

The present disclosure provides fusion proteins comprising Mycobacterium tuberculosis (Mtb) antigens, nucleic acid molecules encoding the same, vectors comprising nucleic acid molecules, compositions comprising the same, and methods of eliciting an immune response against tuberculosis.

Claims (36)

1. A fusion protein comprising at least four or five Mycobacterium tuberculosis (Mtb) antigens, wherein the Mtb antigens are chosen from Rv1009, Rv3136, Rv3615c, Rv2628, Rv2034, and Rv3136 N-terminus, and wherein the fusion protein is chosen from:

Rv1009-Rv2628-Rv3615c-Rv3136;

Rv1009-Rv2034-Rv2628-Rv3615c-Rv3136;

Rv1009-Rv3615c-Rv2034-Rv2628;

Rv3615c-Rv2034-Rv2628-Rv1009;

Rv2034-Rv3615c-Rv2628-Rv3136;

Rv3136-Rv2628-Rv3615c-Rv2034;

Rv1009-Rv3136Nt-Rv2628-Rv2034-Rv3615c;

Rv2034-Rv3615c-Rv3136Nt-Rv2628-Rv1009;

Rv3615c-Rv2628-Rv1009-Rv3136Nt-Rv2034;

Rv1009-Rv2628-Rv3136Nt-Rv2034-Rv3615c;

Rv2628-Rv3136Nt-Rv1009-Rv2034-Rv3615c; and

Rv2628-Rv3136Nt-Rv2034-Rv3615c-Rv1009.

2. The fusion protein according to claim 1 wherein:

Rv1009 comprises the amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:4;

Rv3136 comprises the amino acid sequence set forth in SEQ ID NO:8;

Rv3615c comprises the amino acid sequence set forth in SEQ ID NO:11;

Rv2628 comprises the amino acid sequence set forth in SEQ ID NO:14;

Rv2034 comprises the amino acid sequence set forth in SEQ ID NO:16; and

Rv3136Nt comprises the amino acid sequence set forth in SEQ ID NO:18.

3. The fusion protein according to claim 1 wherein:

Rv1009-Rv2628-Rv3615c-Rv3136 comprises the amino acid sequence set forth in SEQ ID NO:43;

Rv1009-Rv2034-Rv2628-Rv3615c-Rv3136 comprises the amino acid sequence set forth in SEQ ID NO:45;

Rv1009-Rv3615c-Rv2034-Rv2628 comprises the amino acid sequence set forth in SEQ ID NO:51;

Rv3615c-Rv2034-Rv2628-Rv1009 comprises the amino acid sequence set forth in SEQ ID NO:53;

Rv2034-Rv3615c-Rv2628-Rv3136 comprises the amino acid sequence set forth in SEQ ID NO:55;

Rv3136-Rv2628-Rv3615c-Rv2034 comprises the amino acid sequence set forth in SEQ ID NO:57;

Rv1009-Rv3136Nt-Rv2628-Rv2034-Rv3615c comprises the amino acid sequence set forth in SEQ ID NO:59;

Rv2034-Rv3615c-Rv3136Nt-Rv2628-Rv1009 comprises the amino acid sequence set forth in SEQ ID NO:61;

Rv3615c-Rv2628-Rv1009-Rv3136Nt-Rv2034 comprises the amino acid sequence set forth in SEQ ID NO:63;

Rv1009-Rv2628-Rv3136Nt-Rv2034-Rv3615c comprises the amino acid sequence set forth in SEQ ID NO:71;

Rv2628-Rv3136Nt-Rv1009-Rv2034-Rv3615c comprises the amino acid sequence set forth in SEQ ID NO:73; and

Rv2628-Rv3136Nt-Rv2034-Rv3615c-Rv1009 comprises the amino acid sequence set forth in SEQ ID NO:75.

4. A pharmaceutical composition comprising the fusion protein according to claim 1 and a pharmaceutically acceptable carrier.

5. A method of eliciting an immune response against Mycobacterium tuberculosis in a mammal comprising administering to the mammal an immunologically sufficient amount of the fusion protein according to claim 1 .

6. The method of claim 5 wherein the fusion protein is administered to the mammal in the form of an aerosol.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 24, 2018
From: AERAS
To: INTERNATIONAL AIDS VACCINE INITIATIVE, INC.
Reel/Frame 047294/0267 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 30, 2017
From: ANANTHA, RAVI P.; EVANS, THOMAS G.; BONAVIA, AURELIO M.
To: AERAS
Reel/Frame 043451/0591 →
Continuity (2)
Provisional Application 62350837 · Jun 16, 2016
Related Publication 20170362284A1 · Dec 21, 2017