Microfluidic system
The present invention provides microfluidic technology enabling rapid and economical manipulation of reactions on the femtoliter to microliter scale.
1. A microfluidic system for forming and collecting a plurality of plugs, the system comprising:
a microfluidic device comprising at least a first channel and a second channel intersecting with each other at a junction and a microchannel extending from the junction and having an outlet downstream of the junction, wherein the first channel includes at least a first plug fluid within and the second channel includes a carrier fluid that is immiscible with the first plug fluid within the second channel;
a detachable holding component comprising a microchannel having an inlet and an outlet, wherein the inlet is detachably couplable to the outlet of the microchannel of the microfluidic device; and
a pump operably couplable to the holding component and configured to apply a suction force thereto such that, upon application of a suction force thereto, the suction force is further applied to the microchannel of the microfluidic device to thereby cause at least the first plug fluid and immiscible carrier fluid to flow through the first and second channels, respectively and toward the junction to thereby cause formation of a plurality of plugs of the first plug fluid in the immiscible carrier fluid at the junction and subsequent transfer of the plurality of plugs from the microfluidic device to the holding component, wherein each plug is separated from an immediately adjacent plug within the microchannel of the holding component.
2. The microfluidic system of claim 1 , wherein the holding component comprises coiled tubing.
3. The microfluidic system of claim 2 , wherein the tubing comprises a material selected from the group consisting of glass, silicon, a silicone elastomer, and a polymer.
4. The microfluidic system of claim 3 , wherein the tubing material comprises polypropylene or polyethylene.
5. The microfluidic system of claim 4 , wherein the tubing comprises polydimethylsiloxane (PDMS).
6. The microfluidic system of claim 5 , wherein the holding component is a PDMS-based preformed cartridge.
7. The microfluidic system of claim 3 , wherein at least a portion of the tubing is transparent.
8. The microfluidic system of claim 1 , further comprising a receiving component comprising a microchannel having an inlet, wherein the outlet of the microchannel of the holding component is detachably couplable to the inlet of the microchannel of the receiving component.