Methods and compositions for identification of source of microbial contamination in a sample
View Patent ↗Herein are described 1058 different bacterial taxa that were unique to either human, grazing mammal, or bird fecal wastes. These identified taxa can serve as specific identifier taxa for these sources in environmental waters. Two field tests in marine waters demonstrate the capacity of phylogenetic microarray analysis to track multiple sources with one test.
1. An analysis system for detecting the source of fecal bacteria contamination in an environmental sample, comprising probes capable of detecting at least 10% of the source-specific taxa set forth in Table 1 (bird), Table 2 (grazer), Table 3 (sewage), or a combination thereof, wherein each of said probes is about 16 to about 35 nucleotides in length, and wherein the analysis system consists of about 10 5 to about 10 8 of said probes.
2. The analysis system of claim 1 , comprising probes capable of detecting at least 20% of the source-specific taxa set forth in Table 1 (bird), Table 2 (grazer), Table 3 (sewage), or a combination thereof.
3. The analysis system of claim 1 , wherein the probes are capable of detecting at least 10% of the source-specific taxa set forth in Table 1 (bird).
4. The analysis system of claim 1 , wherein the probes are capable of detecting at least 10% of the source-specific taxa set forth in Table 2 (grazer).
5. The analysis system of claim 1 , wherein the probes are capable of detecting at least 10% of the source-specific taxa set forth in Table 3 (sewage).
6. The analysis system of claim 1 , wherein the probes are capable of detecting at least 10% of each of the source-specific taxa set forth in Table 1 (bird), Table 2 (grazer), and Table 3 (sewage).
7. The analysis systems of claim 1 , wherein the probes are probes for microarray probe hybridization.
8. The analysis system of claim 1 , wherein the analysis system comprises a microarray, a bead array, a through-hole array, or a well array.
9. The analysis system of claim 1 , wherein the environmental sample has fecal contamination from bird feces.
10. The analysis system of claim 1 , wherein the environmental sample has fecal contamination from grazer feces.
11. The analysis system of claim 1 , wherein the environmental sample has fecal contamination from human feces and/or sewage.
12. The analysis system of claim 1 , wherein the environmental sample is an air sample, a water sample, a soil sample, or a mixture thereof.
13. The analysis system of claim 1 , wherein the environmental sample is a solid sample.
14. The analysis system of claim 1 , wherein the probes are capable of hybridizing to a target sequence of at least 15% of the source-specific taxa set forth in Table 1 (bird), Table 2 (grazer), Table 3 (sewage), or a combination thereof.
15. The analysis system of claim 14 , wherein the target sequence is a 16S rRNA gene sequence.
16. The analysis system of claim 1 , wherein the analysis system comprises about 10 5 to about 10 7 of said probes.
17. The analysis system of claim 1 , further comprising 1 to about 500000 control probes.
18. The analysis system of claim 1 , wherein each of said probes is about 17 to about 35 nucleotides in length.
19. The analysis system of claim 1 , wherein each of said probes is about 18 to about 35 nucleotides in length.