Wheat with increased resistant starch levels
A series of independent human-induced non-transgenic mutations found at one or more of the SBEII genes of wheat; wheat plants having these mutations in one or more of their SBEII genes; and a method of creating and finding similar and/or additional mutations of SBEII by screening pooled and/or individual wheat plants. The seeds and flour from the wheat plants of the present invention exhibit an increase in amylose and resistant starch without having the inclusion of foreign nucleic acids in their genomes. Additionally, the wheat plants of the present invention exhibit altered SBEII activity without having the inclusion of foreign nucleic acids in their genomes.
1. A wheat plant of the species Triticum aestivum comprising one or more homozygous severe mutations in both SBEIIa alleles of each SBEIIa gene of the A, B, and D genomes, wherein said wheat plant produces grain that germinates, and further wherein grain from said wheat plant has an increased amylose level as compared to grain from a wild type wheat plant.
2. Wheat grain from the wheat plant of claim 1 .
3. Flour comprising a cell of the wheat grain of claim 2 .
4. A food or beverage product comprising a cell of the wheat plant of claim 1 .
5. A wheat grain, plant part, or progeny thereof from the wheat plant of claim 1 .
6. The wheat plant according to claim 1 further comprising:
one or more mutations in both SBEIIb alleles of the SBEIIb gene of only one of the A, B, or D genome.
7. The wheat plant according to claim 1 further comprising:
one or more mutations in both SBEIIb alleles of the SBEIIb gene of only two of the A, B, and D genomes.
8. The wheat plant according to claim 1 further comprising:
one or more mutations in both SBEIIb alleles of each SBEIIb gene of the A, B, and D genomes.