Antibodies against TIM3 and uses thereof
Provided herein are antibodies, or antigen-binding portions thereof, that bind to T-cell immunoglobulin and mucin-domain containing-3 (TIM3) protein. Also provided are uses of these antibodies, or antigen-binding portions thereof, in therapeutic applications, such as treatment of cancer. Further provided are cells that produce the antibodies, or antigen-binding portions thereof, polynucleotides encoding the heavy and/or light chain regions of the antibodies, or antigen-binding portions thereof, and vectors comprising the polynucleotides encoding the heavy and/or light chain regions of the antibodies, or antigen-binding portions thereof.
1. An isolated antibody, which binds to human TIM3, comprising
a heavy chain CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 41, a heavy chain CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 122, and a heavy chain CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 126, and a light chain CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 64, a light chain CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 66, and a light chain CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 68.
2. The antibody of claim 1 , comprising a heavy chain variable region (VH) and a light chain variable region (VL), wherein the VH comprises the amino acid sequence set forth in SEQ ID NO: 364 and the VL comprises the amino acid sequence set forth in SEQ ID NO: 60.
3. The antibody of claim 1 , wherein the antibody is selected from the group consisting of an IgG1, an IgG2, an IgG3, an IgG4, and a variant thereof.
4. The antibody of claim 1 , comprising an effectorless IgG1 Fc.
5. The antibody of claim 1 , comprising a heavy chain and a light chain, wherein the heavy chain comprises the amino acid sequence set forth in SEQ ID NO: 349 and the light chain comprises the amino acid sequence set forth in SEQ ID NO: 29.
6. The antibody of claim 1 , comprising a heavy chain and a light chain, wherein the heavy chain comprises the amino acid sequence set forth in SEQ ID NO: 351 and the light chain comprises the amino acid sequence set forth in SEQ ID NO: 29.
7. The antibody of claim 1 , comprising a heavy chain and a light chain, wherein the heavy chain comprises the amino acid sequence set forth in SEQ ID NO: 353 and the light chain comprises the amino acid sequence set forth in SEQ ID NO: 29.
8. A composition comprising the antibody of claim 1 , and a carrier.
9. The antibody of claim 1 , comprising a heavy chain and a light chain, wherein the heavy chain comprises the amino acid sequence set forth in SEQ ID NO: 350 and the light chain comprises the amino acid sequence set forth in SEQ ID NO: 29.
10. The antibody of claim 1 , comprising a heavy chain and a light chain, wherein the heavy chain comprises the amino acid sequence set forth in SEQ ID NO: 352 and the light chain comprises the amino acid sequence set forth in SEQ ID NO: 29.
11. The antibody of claim 1 , comprising a heavy chain and a light chain, wherein the heavy chain comprises the amino acid sequence set forth in SEQ ID NO: 354 and the light chain comprises the amino acid sequence set forth in SEQ ID NO: 29.
12. A composition comprising the antibody of claim 2 , and a carrier.
13. A composition comprising the antibody of claim 5 , and a carrier.
14. A composition comprising the antibody of claim 6 , and a carrier.
15. A composition comprising the antibody of claim 7 , and a carrier.
16. A composition comprising the antibody of claim 9 , and a carrier.
17. A composition comprising the antibody of claim 10 , and a carrier.
18. A composition comprising the antibody of claim 11 , and a carrier.
19. The antibody of claim 2 , wherein the antibody is selected from the group consisting of an IgG1, an IgG2, an IgG3, an IgG4, and a variant thereof.
20. The antibody of claim 2 , comprising an effectorless IgG1 Fc.
21. A composition comprising the antibody of claim 19 , and a carrier.
22. A composition comprising the antibody of claim 20 , and a carrier.