Single nucleotide polymorphisms and community-associated methicillin-resistant
The present invention is based on the discovery of polymorphisms (SNPs) in the penicillin binding protein (pbp3) gene in Staphylococcus aureus . The presence of G88A and/or G2047A SNPs provides an accurate, reliable biomarker for the presence of Methicillin Resistant Staphylococcus aureus (MRSA), specifically the community-associated MRSA (CA-MRSA). The present invention provides reagents used for detecting the SNPs as well as methods of identifying and using these variants to screen subjects for presence of CA-MRSA. The methods involve isolating a biological sample from a mammal (preferably a human) and testing for the presence of a SNP in the pbp3 gene which is associated with CA-MRSA.
1. A kit for detecting the presence of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) in a human, comprising:
(a) an allele-specific primer pair comprising a forward primer and a reverse primer for amplifying a region containing a SNP selected from the group consisting of G88A and G2047A of the penicillin binding protein 3 gene to produce an amplicon; and
(b) an instruction of using said allele-specific primer pair in a PCR to determine the presence of said SNP, wherein the presence of said SNP is indicative of the presence of CA-MRSA and said primer pair comprises a forward primer of SEQ ID No. 26 and a reverse primer of SEQ ID No. 36.