COMPOSITION DERIVED FROM MAMMALIAN UMBILICAL CORD AND WHARTONS JELLY FOR USE IN THERAPEUTIC AND REGENERATIVE APPLICATIONS
A method of preparation of a solution used for therapeutic and regenerative applications having a concentration of at least about .05 microgram of total protein extract is disclosed. The method comprises the steps of: collecting an umbilical cord; incubating the umbilical cord in a solution containing antibiotics for sterilization; washing the umbilical cord in a buffered salt solution; homogenizing the umbilical cord in the buffered salt solution so as to produce an aqueous solution including the buffered salt solution; applying a sonicator at ultrasonic frequencies to the aqueous solution to further disrupt the aqueous solution; placing the aqueous solution into a centrifuge to separate the aqueous solution into a soluble component and a non-soluble component; filtrating and discarding the non-soluble component of the aqueous solution from the centrifuge through a 0.2 micron filter; and measuring protein concentration of the soluble component to insure at least about 0.05 microgram of total protein extract.
1. A method of preparation of a solution used for therapeutic and regenerative applications having a concentration of at least about 0.05 micrograms of total protein extract, the method comprising the steps of:
a) collecting an umbilical cord;
b) incubating the umbilical cord in a solution containing antibiotics for sterilization at a temperature not exceeding 70 degrees Centigrade;
c) washing the umbilical cord in a buffered salt solution;
d) homogenizing the umbilical cord in the buffered salt solution so as to produce an aqueous solution including the buffered salt solution;
e) applying a sonicator at ultrasonic frequencies to the aqueous solution to further disrupt the aqueous solution;
f) placing the aqueous solution into a centrifuge at about 1,000-15,000 rpm to separate the aqueous solution into a soluble component and a non-soluble component;
g) filtrating and discarding the non-soluble component of the aqueous solution from the centrifuge through a 0.2 micron filter; and
h) measuring protein concentration of the soluble component to insure at least about 0.05 microgram of total protein extract.
2 . The method of claim 1 , wherein the solution used for therapeutic and regenerative applications has a concentration of at least about 2 micrograms of total protein extract.
3 . The method of claim 2 , wherein the step of collecting the umbilical cord further comprises collecting an umbilical cord from a mammal at the time of birth of an offspring of the mammal.
4 . The method of claim 3 , wherein the step of incubating the umbilical cord further comprises incubating the umbilical cord in a solution containing antibiotics for sterilization at a temperature not exceeding 50 degrees Centigrade.
5 . The method of claim 4 , wherein the step of homogenizing the umbilical cord further comprises breaking up the umbilical cord in the buffered salt solution using a blender so as to produce an aqueous solution including minced tissue less than 1 mm in size.
6 . The method of claim 5 , wherein the step of placing the aqueous solution into a centrifuge further comprises placing the aqueous solution into a centrifuge at about 3,000-10,000 rpm.
7 . The method of claim 6 , wherein the step of measuring the protein concentration of the soluble component further comprises measuring protein concentration of the soluble component to insure at least about 2 micrograms of total protein extract.
8 . The method of claim 1 , further comprising mixing the soluble component with liposomes, resulting in a mix.
9 . The method of claim 8 , further comprising mixing the mix with cream as a vehicle for application to a mammal, so as to produce a mixed cream.
10 . The method of claim 9 , further comprising depositing the mixed cream in a container for transport to users.
11 . A method of preparation of a cream for topical application having a concentration of at least about 2 micrograms of total protein extract, the method comprising the steps of:
a) collecting a mammalian umbilical cord;
b) incubating the umbilical cord in a solution containing antibiotics for sterilization at a temperature not exceeding 70 degrees Centigrade;
c) washing the umbilical cord in a buffered salt solution;
d) homogenizing the umbilical cord in the buffered salt solution so as to produce an aqueous solution including the buffered salt solution;
e) applying a sonicator at ultrasonic frequencies to the aqueous solution to further disrupt the aqueous solution;
f) placing the aqueous solution into a centrifuge at 1,000-15,000 rpm to separate the aqueous solution into a soluble component and a non-soluble component;
g) filtrating and discarding the non-soluble component of the aqueous solution from the centrifuge through a 0.2 micron filter; and
h) measuring protein concentration of the soluble component to insure at last about 1 microgram of total protein extract.
12 . The method of claim 11 , wherein the solution used for topical application has a concentration of at least about 2 micrograms of total protein extract.
13 . The method of claim 12 , wherein the step of collecting the umbilical cord further comprises collecting an umbilical cord from a mammal at the time of birth of an offspring of the mammal.
14 . The method of claim 13 , wherein the step of incubating the umbilical cord further comprises incubating the umbilical cord in a solution containing antibiotics for sterilization at a temperature not exceeding 50 degrees Centigrade.
15 . The method of claim 14 , wherein the step of homogenizing the umbilical cord further comprises breaking up the umbilical cord in the buffered salt solution using a blender so as to produce an aqueous solution including minced tissue less than 1 mm in size.
16 . The method of claim 15 , wherein the step of placing the an aqueous solution into a centrifuge further comprises placing the an aqueous solution into a centrifuge at about 3,000-10,000 rpm.
17 . The method of claim 16 , wherein the step of measuring the protein concentration of the soluble component further comprises measuring protein concentration of the soluble component to insure at last about 2 micrograms of total protein extract.
18 . The method of claim 11 , further comprising mixing the soluble component with liposomes, resulting in a mix.
19 . The method of claim 18 , further comprising mixing the mix with cream as a vehicle for application to a mammal, so as to produce a mixed cream.
20 . The method of claim 19 , further comprising depositing the mixed cream in a container for transport to users.