Methods of use for intermediate filament protein probes
The present disclosure relates to compounds and methods for labeling and/or detecting proteins in vivo, and methods for probing mitochondrial structure and/or dynamics.
1. A method of detecting a gliotic condition in a subject, comprising:
administering to the subject a compound of formula (I)
wherein:
A is a fluorophore;
obtaining a tissue sample from the subject; and
detecting the compound of formula (I) in the tissue sample using fluorescence or near-infrared imaging, thereby detecting a gliotic condition in the subject;
wherein the gliotic condition is selected from the group consisting of choroidal neovascularization, diabetic retinopathy, glaucoma, and vitreoretinopathy.
2. The method of claim 1 , wherein the subject is a mammal.
3. The method of claim 1 , wherein the compound binds a type III intermediate filament protein in an eye of the subject.
4. The method of claim 1 , wherein A is:
wherein represents the point of attachment to the nitrogen atom in formula (I).
5. The method of claim 1 , wherein A is a fluorophore selected from the group consisting of a fluorescein, a rhodamine, a coumarin, a pyrene, a cyanine, a squaraine, and a boron-dipyrromethene.
6. The method of claim 1 , wherein A is a boron-dipyrromethene moiety derived from a boron-dipyrromethene N-hydroxysuccinimidyl ester.
7. The method of claim 1 , wherein A is selected from the group consisting of:
wherein represents the point of attachment to the nitrogen atom in formula (I).
8. The method of claim 1 , wherein A is a near-IR dye selected from the group consisting of a cyanine, a squaraine, a phthalocyanine, a porphyrin, and a boron-dipyrromethene.
9. The method of claim 8 , wherein the near-IR dye is a cyanine dye derived from a cyanine N-hydroxysuccinimidyl ester.
10. The method of claim 8 , wherein A is:
wherein represents the point of attachment to the nitrogen atom in formula (I).
11. The method of claim 1 , wherein the compound of formula (I) is administered intraperitoneally.
12. The method of claim 1 , wherein the tissue is a fibrotic tissue or gliotic tissue.
13. The method of claim 3 , wherein the type III intermediate filament protein is vimentin or glial fibrillary acidic protein.